Abstract
It is now widely recognised that the giant polytene chromosomes found in terminally differentiated tissues of certain organisms, most notably amongst the Diptera, offer unique opportunities for correlated cytological and biochemical approaches to the study of gene activity and its regulation (Ashburner, 1970). Unfortunately, until the advent of mass isolation techniques (e.g. Boyd et al, 1968; Zweidler and Cohen, 1971), tissues with polytene chromosomes have been better known to cytologists than to biochemists. One consequence has sometimes been the selection of experimental material for studies of gene activity on the basis of favourable chromosome banding and chromosome continuity. Such studies have proved especially helpful in revealing the relation of local chromosome dispersion (“puffing”) to gene activity (Beermann, 1952; Ashburner, 1970). On the other hand, the occurrence and functional significance of larger scale chromosome dispersion, involving whole blocks of band-interband sequences, may escape attention if a criterion which ensures a relatively low level of gene read-out is employed in choosing tissues for investigation (Thomson, 1969).
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References
Ashburner, M. (1970) Adv. Insect Physiol. 7, 1
Beermann, W. (1952) Chromosoma 5, 139
Beermann, W. (1956) Cold Spring Harbor Symp. Quant. Biol 21, 217
Bier, K. (1959) Chromosoma 10, 619
Boyd, J. B., Berendes, H. A. and Boyd, H. (1968) J. Cell Biol. 38, 369
Busch, H. and Smetana, K. (1970) The Nucleolus, 1st edn., p. 35, Academic Press, London and New York
Cameron, I. L. and Prescott, D. M. (1963) Exptl Cell Res. 30, 609
Estable, C. and Sotelo, J. R. (1952) Stain Technol. 27, 307
Harris, H. (1970) Nucleus and Cytoplasm, 2nd edn., p. 120, Oxford University Press, London
Jacob, J. and Danieli, G. A. (1970) Experientia 26, 1390
Kinnear, J. F., Martin, M-D., Thomson, J. A. and Neufeld, G. J. (1968) Aust. J. Biol Sci. 21, 1033
Kinnear, J. F., Martin, M-D. and Thomson, J. A. (1971) Aust. J. Biol Sci. 24, 275
Kosswig, C. and Shengiin, A. (1947) J. Hered. 38, 235
Love, R. and Liles, R. H. (1959) J. Histochem. Cytochem. 7, 164
Love, R. and Suskind, R. G. (1961) Exptl. Cell Res. 22, 193
Martin, M-D., Kinnear, J. F. and Thomson, J. A. (1969) Aust. J. Biol Sci. 22, 935
Painter, T. S. and Biesele, J. J. (1966) Proc. Nat. Acad. Sci. U.S. 56, 1920
Pardue, M. L., Gerbi, S. A., Eckhardt, R. A. and Gall, J. G. (1970) Chromosoma 29, 268
Price, G. M. (1966) J. Insect Physiol 12, 731
Ringborg, U. and Rydlander, L. (1971) J. Cell Biol 51, 355
Tata, J. R. (1968) Nature (London) 219, 331
Thomson, J. A. (1969) Currents mod. Biol 2, 333
Thomson, J. A. and Gunson, M. M. (1970) Chromosoma 30, 193
Zweidler, A. and Cohen, L. H. (1971) J. Cell Biol 51, 240
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Thomson, J.A. (1973). Patterns of Gene Activity in Larval Tissues of the Blowfly, Calliphora . In: Pollak, J.K., Lee, J.W. (eds) The Biochemistry of Gene Expression in Higher Organisms. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-2550-8_19
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DOI: https://doi.org/10.1007/978-94-010-2550-8_19
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