Optical Studies on T4 Gene Product 32 Protein DNA Interaction

  • Marcos F. Maestre
  • Jan Greve
  • Junko Hosoda
Conference paper
Part of the The Jerusalem Symposia on Quantum Chemistry and Biochemistry book series (JSQC, volume 10)


Bacteriophage T4 gene 32 coded protein (gp32) was isolated by Alberts and coworkers (1,2,3). It binds tightly, cooperatively and preferentially to single-stranded DNA. It facilitates both the denaturation and the renaturation of DNA. Genetic studies have demonstrated that this protein is essential for genetic recombination (4), DNA replication (5), repair of radiation damaged DNA (6), and protection of replicating T4 chromosome from degradative activities (7,8). In vitro, gp32 enhances the rate at which T4 DNA polymerase utilizes single-stranded DNA temperates by 5 to 10-fold (9). Gp32 is also one of the essential components of the reconstructed DNA replicating apparatus (11).


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Copyright information

© Springer Science+Business Media Dordrecht 1977

Authors and Affiliations

  • Marcos F. Maestre
    • 1
  • Jan Greve
    • 1
  • Junko Hosoda
    • 1
  1. 1.Space Sciences Laboratory BerkeleyUniversity of CaliforniaUSA

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