Single Cell Fluorescence Imaging to Investigate Calcium Signaling in Primary Cultured Neurones
This chapter will discuss the use of single cell fluorescence imaging (excluding confocal imaging which is discussed in Nadal and Soria’s chapter in this book) to investigate calcium signalling in primary cultured neurones with particular emphasis on cerebellar granule cell neuronal cultures (CGCs).
KeywordsCalcium Channel Cerebellar Granule Cerebellar Granule Cell Calcium Response Cerebellar Granule Neuron
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- del Río, E., Mclaughlin, M., Downes, CP. and Nicholls, D.G., 1999, Differential coupling of G-protein-linked receptors to calcium mobilization through inositol (1,4,5) trisphosphate or ryanodine receptors in cerebellar granule cells in primary culture, Eur. J. Neurosci. 11, 3015–3022.PubMedCrossRefGoogle Scholar
- Wiley, J.W., Moses, H.C., Gross, R.A. and Macdonald, R.L., 1997, Dynorphin A-mediated reduction in multiple calcium currents involves a G(0 alpha)-subtype G protein in rat primary afferent neurons, J. Neurophys. 77, 1338–1348.Google Scholar