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Time Resolved Excitation Emission (TEE) Spectra: A New Screening Tool Based on Multidimensional Fluorescence Spectroscopy

  • U. Panne
  • R. Düsing
  • R. Niessner

Abstract

Fluorescence spectroscopy is a sensitive method for the detection of anthropogenic fluorescent pollutants, e.g., oils and fuels or polycyclic aromatic hydrocarbons (PAH), in our environment. However, for applications with fibre optical sensor systems static emission spectra do not always provide sufficient information due to their broad and featureless character. In the case of complex mixtures of fluorophores and chromophores, the fluorescence emission spectra and fluorescence lifetime show a pronounced dependence from the excitation wavelength. Hence additional orthogonal dimensions such as decay time and variable excitation wavelengths are needed. The objective of this work is to utilize a combination of static and dynamic fluorescence spectroscopy, i.e., time resolved excitation emission (TEE) fluorescence spectroscopy, for a fibre optical sensor system. The obtained trilinear data arrays, i.e., fluorescence intensity vs. time, excitation, and emission wavelength, provide then a multitude of information about the excited molecules and their local environment.

Keywords

Polycyclic Aromatic Hydrocarbon Emission Spectrum Fluorescence Spectroscopy Fluorescence Emission Spectrum Stimulate Raman Scattering 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Berlman, I. B. (1965): Handbook of Fluorescence Spectra of Aromatic Molecules. London (Academic Press).Google Scholar
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  4. Panne, U., Dicke, C., Duesing, R., Niessner, R. & Bidoglio, G. (2000): “Stimulated raman scattering as an excitation source for time-resolved excitation-emission fluorescence spectroscopy with fiber-optical sensors.” — Appl. Spectrosc. 54: 536-547.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2002

Authors and Affiliations

  • U. Panne
    • 1
  • R. Düsing
    • 1
  • R. Niessner
    • 1
  1. 1.Institute of HydrochemistryTechnical University of MunichMunichGermany

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