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Micromachined Hollow Needle with Integrated Pressure Sensor for Precise, Calibrated Injection into Cells and Embryos

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Micro Total Analysis Systems 2002

Abstract

We have developed surface micromachined hollow silicon nitride needles, specifically designed for injection of DNA and proteins into fruit fly embryos. Precisely controlled calibration of the injected volume is possible using an integrated membrane-based pressure sensor with interferometric read out. The membrane can withstand pressures in excess of 3 bar. The excellent mechanical stability of the hollow needle was verified in fruit-fly embryo injection experiments and the injection of water and a solution of GFP (green fluorescent protein) were successfully performed. Flow measurements of air through a needle with channel dimensions: 75 μm x 15 μm x 2.5 μm, resulted in a flow rate of rair = 1.4 nl / (s bar).

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References

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Author information

Authors and Affiliations

  1. Department of Electrical Engineering, Stanford University, USA

    S. Zappe, X. J. Zhang, I. W. Jung, R. W. Bernstein & O. Solgaard

  2. Department of Developmental Biology, Stanford University, USA

    E. E. M. Furlong, M. Fish & M. Scott

Authors
  1. S. Zappe
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  2. X. J. Zhang
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  3. I. W. Jung
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  4. R. W. Bernstein
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  5. E. E. M. Furlong
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  6. M. Fish
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  7. M. Scott
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  8. O. Solgaard
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Editor information

Editors and Affiliations

  1. The University of Tokushima, Tokushima, Japan

    Yoshinobu Baba

  2. Waseda University, Tokyo, Japan

    Shuichi Shoji

  3. University of Twente, Enschede, The Netherlands

    Albert van den Berg

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© 2002 Springer Science+Business Media Dordrecht

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Zappe, S. et al. (2002). Micromachined Hollow Needle with Integrated Pressure Sensor for Precise, Calibrated Injection into Cells and Embryos. In: Baba, Y., Shoji, S., van den Berg, A. (eds) Micro Total Analysis Systems 2002. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0504-3_27

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  • DOI: https://doi.org/10.1007/978-94-010-0504-3_27

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3953-6

  • Online ISBN: 978-94-010-0504-3

  • eBook Packages: Springer Book Archive

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