Skip to main content
SpringerLink
Log in
Book cover

Structure and Dynamics of Confined Polymers pp 85–95Cite as

Protein Translocation Channels in Mitochondria

TIM & TOM Channels

  • Conference paper

  • 320 Accesses

Part of the book series: NATO Science Series ((ASHT,volume 87))

Abstract

Protein translocation across membranes is a fundamental cellular process that is especially vital for mitochondrial biogenesis. More than 95% of all mitochondrial proteins must be imported from the cytoplasm. The protein-translocating channels of mitochondria have many identical properties including conductance, voltage dependence and regulation by synthetic peptides whose sequences mimic the targeting domains of precursors. Viewing protein translocation across membranes as movement of polymers through confined pathways has provided a novel perspective of channel function. These considerations suggest the observed flickering of the channels in the presence of targeting peptides represents an interaction of the peptides with the channel rather than translocation events.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Schatz, G. and Dobberstein, B. (1996) Common principles of protein translocation across membranes. Science 271, 1519–1526.

    Article  ADS  Google Scholar 

  2. Blobel, G. and Dobberstein, B. (1975) Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J. Cell Biol. 67, 835–51.

    Article  Google Scholar 

  3. Simon, S.M. and Blobel, G. (1991) A protein-conducting channel in the endoplasmic reticulum. Cell 65, 371–380.

    Article  Google Scholar 

  4. Simon, S.M. and Blobel, G. (1992) Signal peptides open protein-conducting channels in E. coli. Cell 69, 677–684.

    Google Scholar 

  5. Lohret, T.A., Jensen, R. and Kinnally, K.W. 1997. The Tim23 import protein is required for normal activity of a mitochondrial inner membrane channel. J. Cell Biol. 137, 377–386.

    Article  Google Scholar 

  6. Roise, D. and Schatz, G. (1988) Mitochondrial presequences J. Biol. Chem. 263, 4509–4511.

    Google Scholar 

  7. Allison, D.S. and Schatz, G. (1986) Artificial mitochondrial presequences. Proc. Natl. Acad. Sci. (USA) 83, 9011–9015.

    Article  ADS  Google Scholar 

  8. Glaser, S.M., and Cumsky, M.G. (1990) Localization of a synthetic presequence that blocks protein import into mitochondria. J Biol. Chem. 265, 8817–8822.

    Google Scholar 

  9. Hugosson, M., Andreu, D., Boman, H.G. and Glaser, E. (1994) Eur. J. Biochem. 223, 1027–1033.

    Article  Google Scholar 

  10. Kushnareva, Y.E., Campo, M.L., Kinnally, K.W. and Sokolove, P.M. (1999) Effects of signal presequences and non-signal peptides on mitochondrial permeability and membrane conductance. Arch. Biochem. Biophys. 366, 107–115.

    Article  Google Scholar 

  11. Pfanner, N., Douglas, M. G., Endo, T.. Hoogenraad, N. J.. Jensen, R. E., Meijer, M., Neupert, W., Schatz, G., Schmitz, U. K., Shore, G. C. 1996. Trends in Biochemical Sciences 21, 51–52.

    Google Scholar 

  12. Künkele, K.P., Heins, S., Dembowski, M., Nargang, F.E., Benz, R., Thieffry, M., Walz, J., Lill, R., Nussberger, S., and Neupert, W. (1998) The preprotein translocation channel of the outer membrane of mitochondria. Cell 93, 1009–1019.

    Article  Google Scholar 

  13. Juin, P., Pelleschi, M., Sagné, C., Henry, J.-P., Thieffry, M. and Vallette, F.-M. (1995) Involvement of the peptide sensitive channel in the translocation of basic peptides into mitochondria. Biochim. Biophys. Res. Comm. 211, 92–99.

    Article  Google Scholar 

  14. Kinnally, K.W., Muro, C., and Campo, M.L. (2000) MCC and PSC, the putative protein import channels of mitochondria. J. Bioenerg. Biomemb, 32, 47–54.

    Article  Google Scholar 

  15. Hill, K., Model, K., Ryan, M.T., Dietmeier, K., Martin, F., Wagner, R. and Pfanner N. (1998) TOM40 forms the hydrophilic channel of the mitochondrial import pore for preproteins. Nature (London) 395, 516–21.

    Article  ADS  Google Scholar 

  16. Schulke, N., Sepuri, N.B.V. and Pain, D. (1997) In vivo zippering of inner and outer mitochondrial membranes by a stable translocation intermediate. Proc. Natl. Acad. Sei. (USA) 94, 7314–7319.

    Article  ADS  Google Scholar 

  17. Berthold, J., Bauer, M.F., Schneider, H.-C., Klaus, C., Dietmeir, K., Neupert, W. and Brunner, M. (1995) The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system. Cell 81, 1085–1093

    Article  Google Scholar 

  18. Ryan, K.R., Roxanne, S.L. and Jensen, R. (1998) Characterization of the mitochondrial inner membrane translocase complex: the TIM23p hydrophobic domain interacts with Tim17 but not with the other TIM23p molecules. Molec. and Cell. Biol. 18, 178–187.

    Google Scholar 

  19. Ryan, K.R., and Jensen, R.E. (1993) mas6p can be cross-linked to an arrested precursor and interacts with other proteins during mitochondrial protein import. J Biol. Chem. 268, 23743–23746.

    Google Scholar 

  20. Emtage, J.L.T. and Jensen, R.E. (1993) mas6 encodes an essential inner membrane component of the yeast mitochondrial protein import pathway. J. Cell Biol. 122, 1003–1012.

    Google Scholar 

  21. Gasser, S.M., Daum, G. and Schatz, G. (1982) Import of proteins into mitochondria. Energy-dependent uptake of precursors by isolated mitochondria. J. Biol. Chem. 257, 13034–13041.

    Google Scholar 

  22. Martin, J., Mahlke, K. and Pfanner, N. (1991) Role of an energized inner membrane in mitochondrial protein import. J. Biol. Chem. 266, 18051–18057.

    Google Scholar 

  23. Schleyer, M., Schmidt, B. and Neupert, W. (1982) Requirement of a membrane potential for the posttranslational transfer of proteins into mitochondria. Eur. J. Biochem. 125, 109–116.

    Article  Google Scholar 

  24. Kang, P.-J., Ostermann, J., Shilling, J., Neupert, W., Craig, E.A. and Pfanner, N. (1990) Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins. Nature (London) 348, 137–143.

    Article  ADS  Google Scholar 

  25. Ungermann, C., Guiard, B., Neupert, W. and Cyr, D.M. (1996) The delta psi-and Hsp70/MIM44-dependent reaction cycle driving the early steps of protein import into mitochondria. EMBO J. 15, 735–744.

    Google Scholar 

  26. Schneider, H.C., Berthold, J., Bauer, M.F., Dietmeier, K., Guiard, B., Brunner, M. and Neupert, W. (1994) Mitochondrial hsp70/MIM44 complex facilitates protein import. Nature (London) 371, 768–774.

    Article  ADS  Google Scholar 

  27. Rassow, J., Maarse, A.C., Krainer, E., Kubrich, M., Muller, H., Miejer, M., Craig, E.A. and Pfanner, N. (1994) Mitochondrial protein import: biochemical and genetic evidence for interaction of matrix hsp70 and the inner membrane protein MIM44. J Cell Biol. 127, 1547–1556.

    Article  Google Scholar 

  28. Lohret, T.A. and Kinnally, K.W. (1995) Targeting peptides transiently block a mitochondrial channel. J. Biol. Chem. 270, 15950–15953.

    Article  Google Scholar 

  29. Muro, C., Campo, M.L., Stanley, S., Schatz, G. and Kinnally, K.W. Structure and function studies define essential components of the TIM and TOM channels of the mitochondrial protein import complexes. In preparation.

    Google Scholar 

  30. Kasianowicz, J.J.; Brandin, E.; Branton, D.; Deamer, D.W. (1996) Proc. Natl. Acad. Sci. (USA) 93, 13770–13773.

    Article  ADS  Google Scholar 

  31. Akeson, M., Branton, D. Kasianowicz, J.J. Brandin, E. and Deamer, D.W. (1999) Microsecond time-scale discrimination between polycytidylic acid and polyadenylic acid segments within single RNA molecules, Biophys. J 77, 3227–3233.

    Article  Google Scholar 

  32. Henrickson, S.E, Misakian, M., Robertson, B. and Kasianowicz, J.J.. (2000) Asymmetric driven DNA transport in a nanometer-scale pore. Phys. Rev. Lett. 85, 3057–3060.

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Basic Sciences, New York University College of Dentistry, 345 East 24th St., New York, NY, 10010, USA

    Kathleen W. Kinnally

Authors
  1. Kathleen W. Kinnally
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, USA

    John J. Kasianowicz

  2. Department of Biophysics, Pécs University Medical School, Pécs, Hungary

    Miklós S. Z. Kellermayer

  3. Biophysics Laboratory, Department of Chemistry and Biochemistry, University of California, Santa Cruz, USA

    David W. Deamer

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Springer Science+Business Media Dordrecht

About this paper

Cite this paper

Kinnally, K.W. (2002). Protein Translocation Channels in Mitochondria. In: Kasianowicz, J.J., Kellermayer, M.S.Z., Deamer, D.W. (eds) Structure and Dynamics of Confined Polymers. NATO Science Series, vol 87. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0401-5_5

Download citation

  • DOI: https://doi.org/10.1007/978-94-010-0401-5_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-1-4020-0698-2

  • Online ISBN: 978-94-010-0401-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation