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Phage DNA Transport Across Membranes

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Structure and Dynamics of Confined Polymers

Part of the book series: NATO Science Series ((ASHT,volume 87))

Abstract

Phage nucleic acid transport is atypical among membrane transport and thus poses a fascinating problem: transport is unidirectional, and it concerns a unique molecule the size of which may represent 50 times that of the bacterium. The rate of DNA transport can reach values as high as 3,000 to 4,000 base pairs s-1. This raises many questions which will be addressed in this review. Is there a single mechanism of transport for all types of phage? How does the phage genome overcome the hydrophobic barrier of the host envelope? Is DNA transported as a free molecule or in association with proteins? Is such transport dependent on phage and/or host cell components? What is the driving force for transport? Data will be presented on tailed phage which are the most common type of phage, occurring in over 100 genera of bacteria and for which DNA transport has been the most extensively studied. We will show that it is possible to analyse these mechanisms both in vivo and in vitro by using biochemical as well as biophysical approaches.

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Author information

Authors and Affiliations

  1. Institut de Biochimie et Biophysique Moléculaire et Cellulaire, UMR 8619 CNRS, Université Paris Sud, Bât 430, Orsay cedex, 91405, France

    Lucienne Letellier

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  1. Lucienne Letellier
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Editors and Affiliations

  1. Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, USA

    John J. Kasianowicz

  2. Department of Biophysics, Pécs University Medical School, Pécs, Hungary

    Miklós S. Z. Kellermayer

  3. Biophysics Laboratory, Department of Chemistry and Biochemistry, University of California, Santa Cruz, USA

    David W. Deamer

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Letellier, L. (2002). Phage DNA Transport Across Membranes. In: Kasianowicz, J.J., Kellermayer, M.S.Z., Deamer, D.W. (eds) Structure and Dynamics of Confined Polymers. NATO Science Series, vol 87. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0401-5_2

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  • DOI: https://doi.org/10.1007/978-94-010-0401-5_2

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-1-4020-0698-2

  • Online ISBN: 978-94-010-0401-5

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