Over-Expression of Protein Kinase Bα Enhances Recombinant Protein Expression in Transient Systems

Abstract

As more genes are being identified through genomic techniques, the need to rapidly express recombinant proteins for functional studies has become increasingly acute. Transient expression of recombinant protein using COS-1, CV-1 and 293 cells is widely used to address this need. To improve the robustness of host cells for transient expression, the effect of over-expression of Protein Kinase Bα has been explored. In this report we demonstrate that over-expression of PKBα can improve transient recombinant protein expression 40% to >200% depending on the protein being expressed and the cell line used.