Abstract
The rat pheochromocytoma PC12 cell line is widely used as a neuronal cell model system for the investigation of neurotrophic action of nerve growth factor (NGF) because it can respond to NGF by extending neurites and differentiating into sympathetic neurone-like phenotype (Green and Tischler, 1976). A subline of PC 12 cells (PC12D cells) was found to be capable of responding to NGF in a faster rate. They only take 24–48 h to extend neurite after being exposed to NGF (Katoh-Semba et al., 1987). Accordingly, PC12D cells are ideal for rapid screening of large quantity of samples for NGF-potentiating substance.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Brinton RD, Yamazaki RS (1998) Advances and challenges in the prevention and treatment of Alzheimer’s disease. Pharmaceut. Res. 15: 386–399.
Connor B, Dragunow M (1998) The role of neuronal growth factors in neurodegenerative disorders of the human brain. Brain Res. Rev. 27: 1–39.
Greene LA, Tischler AS (1976) Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424–2428.
Katoh-Semba R, Kitajima S, Yamazaki Y, Sano M (1987) Neuritic growth from a new subline of Pc12 pheochromocytoma cells: cyclic AMP mimics the action of nerve growth factor. J. Neurosci. Res. 17: 36–44.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Tsang, C.K., Sagara, A., Kamei, Y. (2001). Neurite Outgrowth Promoting Substance from a Marine Alga, Sargassum Macrocarpum . In: Lindner-Olsson, E., Chatzissavidou, N., Lüllau, E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0369-8_6
Download citation
DOI: https://doi.org/10.1007/978-94-010-0369-8_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3897-3
Online ISBN: 978-94-010-0369-8
eBook Packages: Springer Book Archive