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Application of Alphavirus Vectors in Drug Discovery

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Animal Cell Technology: From Target to Market

Part of the book series: ESACT Proceedings ((ESACT,volume 1))

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Abstract

Alphavirus vectors have proven useful for various in vitro and in vivo applications. The rapid generation of high-titer recombinant alphavirus particles has resulted in overexpression of topologically different recombinant proteins in a broad range of mammalian host cells. The same replication-deficient alphavirus particles have been subjected to in vivo gene delivery studies. These features have made alphavirus vectors attractive for various approaches in drug discovery. Large-scale production of recombinant G protein-coupled receptors and ligand-gated ion channels has significantly facilitated drug screening procedures and purification of receptor protein for biostructural studies. Modified alphavirus vectors with lower cytotoxicity and a temperature-sensitive phenotype has allowed prolonged survival of host cells and inducible expression studies. Furthermore, vectors have been engineered for functional expression of cDNA libraries and the use of vector for anfisense and ribozyme applications. Preliminary studies in animal models have indicated that alphaviruses are potentially attracdve for gene therapy applications.

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Authors and Affiliations

  1. CNS Department, Hoffmannn-La Roche, Bldg 69/440, CH 4070, Basel, Switzerland

    Dr Kenneth Lundstrom

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  1. Dr Kenneth Lundstrom
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Editors and Affiliations

  1. Metcon Medicin AB, Dalenum 17, Lidingö, Sweden

    Elisabeth Lindner-Olsson

  2. Biovitrum AB, Lindhagensgatan 133, 112 76, Stockholm, Sweden

    Nathalie Chatzissavidou

  3. Astra Zeneca, Building 841, 151 85, Södertälje, Sweden

    Elke Lüllau

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© 2001 Springer Science+Business Media Dordrecht

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Lundstrom, K. (2001). Application of Alphavirus Vectors in Drug Discovery. In: Lindner-Olsson, E., Chatzissavidou, N., Lüllau, E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0369-8_11

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  • DOI: https://doi.org/10.1007/978-94-010-0369-8_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3897-3

  • Online ISBN: 978-94-010-0369-8

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