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Layer-by-Layer Method for Immobilization of Protein Molecules on Biochip Surface

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Frontiers of Multifunctional Nanosystems

Part of the book series: NATO Science Series ((NAII,volume 57))

Abstract

The article presents a Layer-by-Layer method to produce new biochip surfaces on gold or mica for protein binding. The chip surfaces were modified by two polyelectrolytes, poly(sodium 4-styrenesulfonate) and pory(diallyldimethylam- monium chloride), which produce negatively and positively charged surfaces, respectively. Then, the modified surfaces were used for the binding of several proteins, such as bovine serum albumine, apo-transferrine, tissue transglutaminase and SAG (sensitive to apoptosis gene) protein. The proteins were self-assembled from aqueous solution on the modified surfaces and adsorption process has been examined by Surface Plasmon Resonance and Atomic Force Microscopy. Our results suggested that negatively charged surface was preferable for the binding of four proteins, even though two surfaces could be used.

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Author information

Authors and Affiliations

  1. Institute of Chemistry of New Materials, National Academy of Sciences, 220141, Minsk, Belarus

    G. K. Zhavnerko

  2. Biomolecule Research Team, Korea Basic Science Institute, Yeoeun-dong 52, Yusung-Ku, 305-333, Taejon, South Korea

    Sun-Ju Yi, Soo-Mi Kweon & Kwon-Soo Ha

Authors
  1. G. K. Zhavnerko
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  2. Sun-Ju Yi
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  3. Soo-Mi Kweon
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  4. Kwon-Soo Ha
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Editor information

Editors and Affiliations

  1. Kyiv National Taras Shevchenko University, Ukraine

    Eugenia Buzaneva

  2. TU Ilmenau, Institut für Physik/Fachgebiet Chemie, Germany

    Peter Scharff

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© 2002 Springer Science+Business Media Dordrecht

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Zhavnerko, G.K., Yi, SJ., Kweon, SM., Ha, KS. (2002). Layer-by-Layer Method for Immobilization of Protein Molecules on Biochip Surface. In: Buzaneva, E., Scharff, P. (eds) Frontiers of Multifunctional Nanosystems. NATO Science Series, vol 57. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0341-4_7

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  • DOI: https://doi.org/10.1007/978-94-010-0341-4_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-1-4020-0561-9

  • Online ISBN: 978-94-010-0341-4

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