Abstract
A brief review of the physical aspects of the spin labeling technique is given. Emphasis is put on the qualitative description of the phenomena which enable us to obtain information on molecular motion in biological and related systems. The conventional use of nitroxides, saturation transfer spectroscopy and electron spin-electron spin interactions are the three topics discussed.
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References
Spin-labeling, theory and applications. Acad. Press, Ed. L. J. Berliner, Vol. I, 1976, Vol.11 in the press 1979.
A pure spin-label crystal would give rise to a single very broad line due to spin-spin interactions, as indicated in section IV.
In figure 1-c, the distribution function for the 3 lines is represented very crudely by 3 rectangles. The actual distribution is more complicated but this figure at least shows that the 3 nuclear spins play unequivalent roles. This disymmetry is due to the g value.
The exact critical value is 54°7, this is because the distribution functions are not simple rectangles as indicated in figure 3.
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The actual power on the sample is one of the crucial parameter to determine; it requires a good calibration of the system.
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© 1980 D. Reidel Publishing Company, Dordrecht, Holland
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Devaux, P.F., Davoust, J. (1980). Physical Aspects of the Spin Labelling Technique. In: Bertini, I., Drago, R.S. (eds) ESR and NMR of Paramagnetic Species in Biological and Related Systems. Nato Advanced Study Institutes Series, vol 52. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-9524-6_17
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DOI: https://doi.org/10.1007/978-94-009-9524-6_17
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