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Histoenzymology of the Human Ovary: Dehydrogenases Directly Involved in Steroidogenesis

  • Chapter
Biology of the Ovary

Part of the book series: Developments in Obstetrics and Gynecology ((DIOG,volume 2))

Abstract

A close and complex functional relationship exists between the ovary, the hypothalamus and the pituitary gland. In the active reproductive period, this means that most of the different cell types in the human ovary undergo dynamic alterations. These are expressed as changes in growth, differentiation, morphology, biochemical function and endocrine activity of the ovarian compartments, such as follicles, corpora lutea, interstitial gland cells and stroma. There is also a functional interrelationship between different cell types, for example, in response to FSH the granulosa cells of the large antral follicle may produce estrogen by aromatizing androgen synthesized by the theca cells (Fowler et al. 1978; Moon et al. 1978).

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References

  • Altman, F.P., Quantitative dehydrogenase histochemistry with special reference to the pentose shunt dehydrogenases. Prog. Histochem. Cytochem. 4: 225, 1972.

    CAS  Google Scholar 

  • Altman, F.P., Høyer, P.E. and Andersen, H., Dehydrogenase histochemistry of lipid-rich tissues: a tetrazolium chelation technique to improve localisation. Histochem. J. 11: 485, 1979.

    Article  PubMed  CAS  Google Scholar 

  • Amoroso, E.C. and Perry, J.S., Ovarian activity during gestation. In: The ovary, (eds. S. Zuckermann and B.J. Weir), Academic Press, New York, vol. 2, pp. 315–398, 1977.

    Google Scholar 

  • Andersen, H. and Høyer, P.E., Simplified control experiments in the histochemical study of coenzyme-linked dehydrogenases. Histochemistry 38: 71, 1974.

    Article  CAS  Google Scholar 

  • Baillie, A.H., Ferguson, M.M. and Hart, D.McK., Histochemical evidence of steroid metabolism in the human genital ridge. J. Clin. Endocr. 26: 738, 1966.

    Article  PubMed  CAS  Google Scholar 

  • Bara, G. and Anderson, W.A., Fine structural localization of 3ß-hydroxysteroid dehydrogenase in rat corpus luteum. Histochem. J. 5: 437, 1973.

    Article  PubMed  CAS  Google Scholar 

  • Benköel, L., Chamlian, A., Barrat, E. and Laffargue, P., The use of ferrycyanide for the electron microscopic demonstration of dehydrogenases in human steroidogenic cells. J. Histochem. Cytochem. 24: 1194, 1976.

    Article  PubMed  Google Scholar 

  • Bjersing L., Ovarian histochemistry. In: The ovary (eds. S. Zuckerman and B.J. Weir), Academic Press, New York, vol. 1, pp. 303–391, 1977.

    Google Scholar 

  • Brandau, H. and Lehmann, V., Histoenzymatische Untersuchungen an menschlichen Gonaden während der intrauterinen Entwicklung. Z. Geburtshilfe Gynaecol. 173: 233, 1970.

    CAS  Google Scholar 

  • Brandau, H. and Luh, W., Die Histotopik von Oxydoreduktasen des Intermediärstoffwechsels im interstitiellen Gewebe des menschlichen Ovars: ein Beitrag zur funktionellen Morphologie der interstitiellen Zellen. Arch. Gynaekol. 200: 407, 1965.

    Article  CAS  Google Scholar 

  • Brandau, H., Lehmann, V. and Kazancigil, A., Histogramme von Oxydoreduktasen des Kohlenhydratstoffwechsels und von Steroid-Dehydrogenasen im Ovar des Neugeborenen. Gynaecologia 165: 428, 1968.

    PubMed  CAS  Google Scholar 

  • Brandau, H., Brandau, L. and Mestwerdt, W., Endocrine activity in postmenopausal ovaries. Eur. J. Obst. Gyn. Reprod. Biol. 4 (suppl.): S 187, 1974.

    Google Scholar 

  • Brändén, C.-J., Jörnvall, H., Eklund, H. and Furugren, B., Alcohol dehydrogenases. In: The enzymes (ed. P.D. Boyer), Academic Press, New York, vol. 11, pp. 103–190, 1975.

    Google Scholar 

  • Breitenecker, G., Kemeter, P. and Friedrich, F., Further investigations on the maturation and degeneration of human ovarian follicles and their oocytes. Fert. Steril. 29: 336, 1978.

    CAS  Google Scholar 

  • Cavallero, C. and Magrini, U., Histochemical studies on 3ß-hydroxysteroid dehydrogenase and other enzymes in the steroid-secreting structures of human foetus. In: Proceedings of the 2nd international congress on hormonal steroids (eds. L. Martini, F. Fraschini and M. Motta), Excerpta Medica, Amsterdam, pp. 667–674, 1967.

    Google Scholar 

  • Christensen, A.K. and Gillim, S.W., The correlation of fine structure and function in steroid-secreting cells, with emphasis on those of the gonads. In: The gonads (ed. K.W. McKerns), North-Holland Publishing, Amsterdam, pp. 415–488, 1969.

    Google Scholar 

  • Davies, J., Davenport, G.R., Norris, J.L. and Rennie, P.I.C., Histochemical studies of hydroxysteroid dehydrogenase activity in mammalian reproductive tissues. Endocrinol. 78: 667, 1966.

    Article  CAS  Google Scholar 

  • Deane, H.W., Lobel, B.L. and Romney, S.L., Enzymic histochemistry of normal human ovaries of the menstrual cycle, pregnancy, and the early puerperium. Am. J. Obstet. Gynecol. 83: 281, 1962.

    PubMed  CAS  Google Scholar 

  • Dimino, M.J., Differences in mitochondrial steroidogenesis between follicular and luteal tissues of porcine ovaries. Endocrinol. 101: 1844, 1977.

    Article  CAS  Google Scholar 

  • Eastman, A.R. and Neville, A.M., 5-ene-3ß-hydroxysteroid dehydrogenase of human and bovin adrenocortical endoplasmic reticulum: solubilization and fractionation. J. Endocr. 72: 225, 1977.

    Article  PubMed  CAS  Google Scholar 

  • Fowler, R.E., Fox, N.L., Edwards, R.G. and Steptoe, P.C., Steroid production from 17α-hydroxypregnenolone and dehydroepiandrosterone by human granulosa cells in vitro. J. Reprod. Fert. 54: 109, 1978.

    Article  CAS  Google Scholar 

  • Friedrich, F., Breitenecker, G., Salzer, H. and Holzner, J.H., The progesterone content of the fluid and the activity of the steroid-3ß-ol-dehydrogenase within the wall of the ovarian follicles. Acta Endocr. 76: 343, 1974.

    PubMed  CAS  Google Scholar 

  • Garbarsch, C., Andersen, H. and Høyer, P.E., Succinate dehydrogenase activity in the wall of rabbit aorta: the histochemical use of PMS and exogenous coenzyme Q10 as intermediate carriers. Histochemistry 51: 297, 1978.

    Article  Google Scholar 

  • Goldberg, B., Jones, G.E.S. and Borkowf, H.I., A histochemical study of substrate specificity for the steroid 3ß-ol dehydrogenase and isomerase systems in human ovary and testis. J. Histochem. Cytochem. 12: 880, 1964.

    Article  PubMed  CAS  Google Scholar 

  • Goldman, A.S., Yakovac, W.C. and Bongiovanni, A.M., Development of activity of 3ß-hydroxysteroid dehydrogenase in human fetal tissues and in two anencephalic newborns. J. Clin. Endocr. 26: 14, 1966.

    Article  PubMed  CAS  Google Scholar 

  • Gondos, B. and Hobel, C.J., Interstitial cells in the human fetal ovary. Endocrinol. 93: 736, 1973.

    Article  CAS  Google Scholar 

  • Gower, D.B., Biosynthesis of the corticosteroids. In: Biochemistry of steroid hormones (ed. H.L.J. Makin), Blackwell, Oxford, pp. 47–75, 1975.

    Google Scholar 

  • Guraya, S.S., Histochemical study of granulosa and theca interna during follicular development, ovulation, and corpus luteum formation and regression in the human ovary. Am. J. Obstet. Gynecol. 101: 448, 1968.

    PubMed  CAS  Google Scholar 

  • Guraya, S.S., Recent advances in the morphology, histochemistry, biochemistry, and physiology of interstitial gland cells of mammalian ovary. Int. Rev. Cytol. 55: 171, 1978.

    Article  PubMed  CAS  Google Scholar 

  • Hardonk, M.J., A new method for the histochemical demonstration of steroid-producing cells in human tissues. Histochemie 5: 234, 1965.

    Article  PubMed  CAS  Google Scholar 

  • Høyer, P.E., and Andersen, H., Specificity in steroid histochemistry, with special reference to the use of steroid solvents: distribution of 11ß-hydroxysteroid dehydrogenase in kidney and thymus from the mouse. Histochemie 24: 292, 1970.

    PubMed  Google Scholar 

  • Høyer, P.E. and Andersen, H., Histochemistry of 3ß-hydroxysteroid dehydrogenase in rat ovary I: a methodological study. 51: 167, 1977.

    Google Scholar 

  • Høyer, P.E. and Andersen, H., Use of some flavoprotein substitutes as intermediate carriers in the histochemical detection of 3ß- and 20α-hydroxysteroid dehydrogenases of rat ovary. 1979a (in preparation).

    Google Scholar 

  • Høyer, P.E. and Andersen, H., 20α-hydroxysteroid dehydrogenase of rat ovary: a soluble or bound enzyme? 1979b (in preparation).

    Google Scholar 

  • Ikonen, M., Niemi, M., Pesonen, S. and Timonen, S., Histochemical localization of four dehydrogenase systems in human ovary during the menstrual cycle. Acta Endocr. 38: 293, 1961.

    CAS  Google Scholar 

  • Jones, G.E.S., Goldberg, B. and Woodruff, J.D., Histochemistry as a guide for interpretation of cell function. Am. J. Obstet. Gynecol. 100: 76, 1968.

    PubMed  CAS  Google Scholar 

  • Karjalainen, O., The human corpus luteum of pregnancy with reference to the state of the trophoblastic tissue: a histological, histochemical and biochemical study. Acta Obstet. Gynecol. Scand. 47 (suppl. 10): 1, 1968.

    Article  PubMed  CAS  Google Scholar 

  • Koudstaal, J. and Jöbsis, A.C., Enzyme histochemistry of the human ovary. Eur. J. Obst. Gyn. Reprod. Biol. 4 (suppl.): S 51, 1974.

    Article  Google Scholar 

  • Koudstaal, J. Bossenbroek, B. and Hardonk, M.J., An investigation into thecomatosis of the ovary and related tumours by histochemical and enzyme histochemical methods. Eur. J. Cancer 2: 313, 1966.

    PubMed  CAS  Google Scholar 

  • Koudstaal, J., Bossenbroek, B. and Hardonk, M.J., Ovarian tumors investigated by histochemical and enzyme histochemical methods. Am. J. Obstet. Gynecol. 102: 1004, 1968.

    PubMed  CAS  Google Scholar 

  • Kugler, P. and Wrobel, K.-H., Meldola blue: a new electron carrier for the histochemical demonstration of dehydrogenases (SDH, LDH, G-6-PDH). Histochemistry 59: 97, 1978.

    Article  PubMed  CAS  Google Scholar 

  • Mestwerdt, W., Die Follikel-Granulosazellen in Beziehung zur Steroid-Biosynthese in der Periovulationsphase. Fortschr. Med. 6: 361, 1977.

    Google Scholar 

  • Moon, Y.S., Tsang, B.K., Simpson, C. and Armstrong, D.T., 17ß-estradiol biosynthesis in cultured granulosa and thecal cells of human ovarian follicles: stimulation by follicle-stimulating hormone. J. Clin. Endocr. Metab. 47: 263, 1978.

    Article  PubMed  CAS  Google Scholar 

  • Mossman, H.W. and Duke, K.L., Comparative morphology of the mammalian ovary, University of Wisconsin Press, Madison, 1973.

    Google Scholar 

  • Motta, P., Takeva, Z. and Bourneva, V., A histochemical study of â–³ 5-3ß-hydroxysteroid dehydrogenase activity in the interstitial cells of the mammalian ovary. Experientia 26: 1128, 1970.

    Article  PubMed  CAS  Google Scholar 

  • Niemi, M., Ikonen, M., Pesonen, S., Saure, A. and Timonen, S., Histochemistry of the corpus luteum in human ovary. Ann. Chir. Gynaec. Fenn. 54: 339, 1965.

    PubMed  CAS  Google Scholar 

  • Pearse, A.G.E., Histochemistry, theoretical and applied, Churchill-Livingstone, London, 3rd ed., vol. 2, 1972.

    Google Scholar 

  • Peters, H. and McNatty, K.P., The ovary: a correlation of structure and function, Granada (Elek), London 1980.

    Google Scholar 

  • Peters, H., Byskov, A.G. and Grinsted, J., Follicular growth in fetal and prepubertal ovaries of humans and other primates. In: Clinics in endocrinology and metabolism, volume 7(3): Reproductive endocrinology (eds. G.T. Ross and M.B. Lipsett), W.B. Saunders, Philadelphia, pp. 469–485, 1978.

    Google Scholar 

  • Pittaway, D.E., Andersen, R.N. and Givens, J.R., Characterization of human ovarian oestradiol-17ß oxidoreductase activity. Acta Endocr. 85: 624, 1977.

    PubMed  CAS  Google Scholar 

  • Poliak, A., Jones, G.E.S., Goldberg, B., Solomon, D. and Woodruff, J.D., Effect of human chorionic gonadotropin on postmenopausal women: ovarian histochemistry and urinary hormone excretion. Am. J. Obstet. Gynecol. 101: 731, 1968.

    CAS  Google Scholar 

  • Robertson, W.R., A quantitative cytochemical method for the demonstration of â–³ 5, 3ß-hydroxy steroid dehydrogenase activity in unfixed tissue sections of rat ovary. Histochemistry 59: 271, 1979.

    Article  PubMed  CAS  Google Scholar 

  • Schaar, H., Funktionelle Morphologie der Theca interna im Bläschenfollikel des menschlichen Ovars. Acta Anat. 94: 283, 1976.

    Article  PubMed  CAS  Google Scholar 

  • Wiest, W.G. and Kidwell, W.R., The regulation of progesterone secretion by ovarian dehydrogenases. In: The gonads (ed. K.W. McKerns), North-Holland Publishing, Amsterdam, pp. 295–325, 1969.

    Google Scholar 

  • Wilcox, R.B. and Wiest, W.G., Further studies of rat ovarian 20α-hydroxysteroid dehydrogenase. Steroids 7: 395, 1966.

    Article  PubMed  CAS  Google Scholar 

  • Zander, J., Forbes, T.R., von Münstermann, A.M. and Neher, R., â–³ 4-ketopregnen-20α-ol and â–³ 4-ketopregnen-20ß-ol, two naturally-occurring metabolites of progesterone: isolation, identification, biologic activity, and concentration in human tissues. J. Clin. Endocr. 28: 337, 1958.

    Article  Google Scholar 

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Authors
  1. P. E. Høyer
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Editors and Affiliations

  1. Department of Anatomy, Faculty of Medicine, University of Rome, viale Regina Elena 289, Rome, 00161, Italy

    P. M. Motta M.D.

  2. Reproductive Physiology Laboratory, C.S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA

    E. S. E. Hafez Ph.D.

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© 1980 Martinus Nijhoff Publishers bv, The Hague

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Høyer, P.E. (1980). Histoenzymology of the Human Ovary: Dehydrogenases Directly Involved in Steroidogenesis. In: Motta, P.M., Hafez, E.S.E. (eds) Biology of the Ovary. Developments in Obstetrics and Gynecology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8852-1_4

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  • DOI: https://doi.org/10.1007/978-94-009-8852-1_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-8854-5

  • Online ISBN: 978-94-009-8852-1

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