Recovery of Ostertagia from the Bovine Abomasal Mucosa by Immersion in Warm Normal Saline

  • N. E. Downey
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 9)


Theodorides and Actor (1967) reported that immature nematodes could be successfully recovered at necropsy by allowing them to migrate from segments of murine small intestine into a surrounding medium of normal saline. This is the principle underlying the method described here for recovering mainly early fourth stage larvae (EL4) of Ostertagia ostertagi from the abomasal mucosa of cattle. The adoption of a similar procedure for this purpose was reported by Williams et al. (1977). Preliminary estimations of recovery rates achieved by the method were carried out.


Adult Worm Digestion Method Worm Count Mature Worm Murine Small Intestine 
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  1. Herlich, H., 1966. A digestion method for post-mortem recovery of nematodes for ruminants. Proceedings of the Helminthological Society of Washington, 23, 102–103.Google Scholar
  2. Theodorides, V.J. and Actor, P., 1967. Simple method for the recovery and counting of helminths of mice. The Journal of Parasitology, 53, 210.PubMedCrossRefGoogle Scholar
  3. Williams, J.C., Knox, J.W., Sheehan, D. and Fuselier, R.H., 1977. Efficacy of albendazole against inhibited early fourth stage larvae of Ostertagia ostertagi. The Veterinary Record, 101, 484–486.PubMedGoogle Scholar

Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1981

Authors and Affiliations

  • N. E. Downey
    • 1
  1. 1.The Agricultural Institute, DunsineaCastleknock, Co.DublinIreland

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