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Freeze-Fracture of Hepatic Fine Structure Under Normal and Experimental Conditions

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Basic and Clinical Hepatology

Part of the book series: Developments in Gastroenterology ((DIGA,volume 2))

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Abstract

The freeze-fracture method is a special preparatory procedure that is utilized in ultrastructural studies and has been applied to biological and medical investigations for more than twenty years. The historical development as well as theoretical aspects of the method have been reviewed in several previous publications [1–3]. Since its inception, the method has been widely used and continuously improved. A particularly impressive current development is quick freezing whereby tissue can be solidified without perceptible structural changes, allowing electron-microscopic specimen analysis close to or resembling that of the ‘in vivo conditions’ [4].

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Authors
  1. J. Metz
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Editors and Affiliations

  1. Department of Anatomy, Faculty of Medicine, University of Rome, Viale Regina Elena 289, I-00161, Rome, Italy

    Pietro M. Motta M.D., Ph.D

  2. Department of Anatomy, Medical College of Ohio, C.S. 10008, Toledo, Ohio, 43699, USA

    Liberato J. A. DiDio M.D., D.Sc., Ph.D

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© 1982 Martinus Nijhoff Publishers, The Hague

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Metz, J. (1982). Freeze-Fracture of Hepatic Fine Structure Under Normal and Experimental Conditions. In: Motta, P.M., DiDio, L.J.A. (eds) Basic and Clinical Hepatology. Developments in Gastroenterology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8216-1_4

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  • DOI: https://doi.org/10.1007/978-94-009-8216-1_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-8218-5

  • Online ISBN: 978-94-009-8216-1

  • eBook Packages: Springer Book Archive

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