Abstract
Among the advantages of CARS regarding biological applications are its high energy conversion, its insensitivity towards fluorescence and its excellent time resolution (1). These strong points should make resonant CARS spectroscopy a preferred method to obtain Raman spectra of fluoresceing biological molecules. Surprisingly, a modest number of biological applications has been reported in the literature as compared with the much larger number of Raman and resonance Raman studies (2). A reason for this paucity of data may be the fact that CARS is not yet a routine method. It requires a judicious selection of biological systems whose spectra should be susceptible to a reasonable interpretation. This is often impossible to achieve with large molecules, a problem equally encountered in spontaneous Raman spectroscopy. Resonance enhancement is indispensible when CARS is applied to dilute aqueous solutions of biological molecules, whose concentrations should be at least 10-3 — 10-4 m/1 in general.
Keywords
- Pump Beam
- Flavin Adenine Dinucleotide
- Flavin Adenine Dinucleotide
- Resonance Enhancement
- Laser Repetition Rate
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Schneider, F.W. (1982). Biological Applications of Resonance CARS. In: Kiefer, W., Long, D.A. (eds) Non-Linear Raman Spectroscopy and Its Chemical Aplications. NATO Advanced Study Institutes Series, vol 93. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7912-3_20
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DOI: https://doi.org/10.1007/978-94-009-7912-3_20
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