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Nucleic Acids: The Vectors of Life pp 89–99Cite as

Probing for and with Left-Handed DNA: Poly[d(A-br5c)•d(G-T)], a Member of a New Family of Z-Forming DNAs

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Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 16))

Abstract

Left-handed helical conformations can be adopted by the poly[d(A-C)•d(G-U)] family of DNAs bearing at least one methyl or halogen (bromine, iodine) substitution at the pyrimidine C5 heterocyclic position. A representative polynucleotide is poly[d(A-br5C)•d(G-T)]. Elevation of salt concentration and temperature shifts the equilibrium in favor of the left-handed state. The spectral changes (u.v. absorption, c.d., NMR, Raman) accompanying the highly co-operative and reversible R(right)-L(left) transitions are similar to those observed with poly[d(G-C)]. Some, but not all, antibodies raised against the left-handed members of the poly[d(G-C)] family also recognize the new class of polymers, thus establishing the existence of sequence-specific determinants in the binding of certain anti-Z DNA immunoglobulins. The latter have been used to establish the presence of left-handed regions in natural plasmid and viral DNAs and in the giant salivary gland polytene chromosomes of Chironomus thummi thummi. The Z-DNA specific immunofluorescence in fixed polytene chromosomes has been quantitated by laser scanning and photon counting and is particularly intense in certain regions, such as the telomeres.

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Authors and Affiliations

  1. Max Planck Institut für biophysikalische Chemie, Postfach 968, D-3400, Göttingen, Germany

    Thomas M. Jovin, Lawrence P. McIntosh, David A. Zarling & Donna J. Arndt-Jovin

  2. Lehrstuhl für Entwicklungsphysiologie, Universität Göttingen, D-3400, Göttingen, Germany

    Michel Robert-Nicoud

  3. Department of Medical Biochemistry, University of Calgary, Alberta, Canada, T2N 4N1

    Johan H. van de Sande

Authors
  1. Thomas M. Jovin
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  2. Lawrence P. McIntosh
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  3. David A. Zarling
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  4. Donna J. Arndt-Jovin
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  5. Michel Robert-Nicoud
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  6. Johan H. van de Sande
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Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Universite Pierre et Marie Curie (Paris VI), Paris, France

    Bernard Pullman

  2. Department of Chemistry, Tel-Aviv University, Tel-Aviv, Israel

    Joshua Jortner

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© 1983 D. Reidel Publishing Company

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Jovin, T.M., McIntosh, L.P., Zarling, D.A., Arndt-Jovin, D.J., Robert-Nicoud, M., van de Sande, J.H. (1983). Probing for and with Left-Handed DNA: Poly[d(A-br5c)•d(G-T)], a Member of a New Family of Z-Forming DNAs. In: Pullman, B., Jortner, J. (eds) Nucleic Acids: The Vectors of Life. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7225-4_7

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  • DOI: https://doi.org/10.1007/978-94-009-7225-4_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-7227-8

  • Online ISBN: 978-94-009-7225-4

  • eBook Packages: Springer Book Archive

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