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Myocardial cell Properties and Hypertrophy

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Cardiac Left Ventricular Hypertrophy

Part of the book series: Developments in Cardiovascular Medicine ((DICM,volume 33))

Summary

The purpose of this study was to analyze whether and to what extent (i) the force-interval relationship, (ii) the forces-arcomere length relationship and (iii) the force-velocity relationship are modified in acute hypertrophy. Mechanical properties of trabeculae that were dissected from the right ventricle of rats were studied. Three experimental models were used: (i) 3–4 months’ old Wistar rats with normal blood pressure served as controls; (ii) hyperthyroid Wistar rats of 3–4 months of age were studied after two weeks of triiodothyronine injections (50 µg daily); (iii) rats of the same age after 2–4 weeks of hypobaric hypoxia which leads to hypertrophy of the right ventricle as a result of pulmonary hypertension at elevated cardiac output. The muscles were studied in a bath, which was rapidly perfused with modified oxygenated Krebs Henseleit solution at 25 ºC at pH 7.4. Force was measured with a strain gauge. Muscle length was measured and controlled with a servomotor. Sarcomere length was measured and controlled by means of laser diffraction techniques. Mechanical recovery curves in controls and hearts following hypobaric hypoxia showed a rapid rise in the first 600 ms followed by a slow rise to plateau level that was attained at 60 seconds. Mechanical recovery in hyperthyroid muscles showed only the rapid phase of recovery. Force at test intervals between 800 ms and 100 s was 100% of steady-state value. Both passive force (F), sarcomere length (SL) relations and active F-SL relations, studied at external calcium concentration = 2.5 mM were comparable for the three groups. No difference in stress development between the three groups was observed. The influence of Ca++ on the shape of F-SL relations in controls and trabeculae of T3 rats was identical. The F-SL curve at Ca++ = 2.5 mM in trabeculae following hypobaric hypoxia was slightly steeper than in controls. Maximal shortening velocity of the sarcomeres (Vo) was 13.6 ± 3.0 µm/s (mean ± 1 SD) in controls; 17.9 ± 2.1 µ m/s in T3 rats; and 8.62 ± 2.0 µ m/s in muscle of hypobaric hypoxia rats. Results suggest 1) increased capacity and transport rate of Ca++ in structures coupling excitation with contraction in T3 hypertrophy compared to controls and hypertrophy resulting from hypobaric hypoxia; 2) no appreciable effect of hypertrophy on length dependence of activation; 3) changes of Vo in the hypertrophy states correlate with reported changes in myosin isoenzyme composition.

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Authors and Affiliations

  1. Department of Cardiology, Leiden University Hospital, Rijnsburgerweg 10, 2333 AA, Leiden, The Netherlands

    H. E. D. J. Keurs, B. J. M. Ter Mulder & V. J. A. Schouten

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  1. H. E. D. J. Keurs
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  2. B. J. M. Ter Mulder
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  3. V. J. A. Schouten
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Editors and Affiliations

  1. Department of Cardiology, Leiden University Hospital, Rijnsburgerweg 10, 2333 AA, Leiden, The Netherlands

    H. E. D. J. Ter Keurs  & J. J. Schipperheyn  & 

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© 1983 Martinus Nijhoff Publishers

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Keurs, H.E.D.J., Ter Mulder, B.J.M., Schouten, V.J.A. (1983). Myocardial cell Properties and Hypertrophy. In: Ter Keurs, H.E.D.J., Schipperheyn, J.J. (eds) Cardiac Left Ventricular Hypertrophy. Developments in Cardiovascular Medicine, vol 33. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6759-5_5

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  • DOI: https://doi.org/10.1007/978-94-009-6759-5_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-6761-8

  • Online ISBN: 978-94-009-6759-5

  • eBook Packages: Springer Book Archive

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