Abstract
This paper summarizcs a survey of the electrophoretic behavior of the tubulins of 23 species (mostly protists) as well as their reactivity towards 4 anti-tubulin antibodies (raised against two ciliate tubulins and two vertebrate ones). Some generalizations concerning the relative migration rates of α VS β tubulin could be made, in particular the α/β inversion, first described in Physarum was extended to several ciliates. Antivertebrate tubulin antibodies displayed a very broad spectrum of reactions, reacting with virtually all the species tested. They appear to correspond to auto-antibodies no exclusively directed against species specific determinants. In contrast, the two anti-ciliate tubulin antibodies displayed a narrow species specificity reacting only with a limited subset of protists. They were shown to be specific for a small number of immunological determinants present on ciliate tubulins. This allowed a rough evaluation of evolutionary relatedness between the various groups of protists analyzed. The results are discussed within the framework of a number of published phyllogenies and shown to be in striking agreement with some of the schemes.
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References
Adoutte, A., Cohen, J., Hill, A. M., Pantaloni, D., and Beisson, J.: 1982, ‘Biochemical and Immunological Comparison of Paramecium and Vertebrate Tubulins, Biol. Cell 45, 265a.
Cavalier-Smith, T.: 1978, ‘The Evolutionary Origin and Phylogeny of Microtubules, Mitotic Spindles and Eucaryote Flagella’, BioSystems 10, 93–114.
Cavalier-Smith, T.: 1981, ‘The Origin and Early Evolution of the Eucaryotic Cell’, in Carlile, Collins and Moseley (eds), Molecular and Cellular Aspects of Microbial evolution, Society for General Microbiology Ltd. Symposium 32, Cambridge University Press.
Cavalier-Smith, T.: 1983, ‘Symbiosis and Eucaryote Phylogeny: The Simultaneous Diversification of Cilia, Mitochondria And Chloroplasts’, in W. Schwemmler and M. Schenk. (eds), Endocytobiology II, de Gruyter, Berlin, in press.
Clayton, L., Quinlan, R. A., Roobol, A., Pogson, C. I., and Gull, K.: 1980, ‘A Comparison of Tubulins from Mammalian Brain and Physarum polycephalum Using SDS-polyacrylamide Gel Electrophoresis and Peptide Mapping, FEBS Lett 115, 301–305.
Cleveland, D. W., Fischer, S. G., Kirschner, M. W., and Laemmli, L. K.: 1977, ‘Peptide Mapping by Limited Proteolysis in Sodium Dodecyl Sulfate and Analysis by Gel Electrophoresis’, J. Bioi. Chem. 252, 1102–1106.
Cohen, J., Adoutte, A., Grandchamp, S., Houdebine, L. M., and Beisson J.: 1982, ‘Immunocytochemical Study of Microtubular Structures Throughout the Cell Cycle of Paramecium’, Biol. Cell 44, 35–44.
Dodge, J. D.: 1979, ‘The Phytoflagellates: Fine Structure and Phylogeny’, in M. Levandowsky and S. H. Hutner (eds), Biochemistry and Physiology of Protozoa, Acad. Press, New-York, pp. 7–57.
Herzog, M., von Boletzky, S., and Soyer, M. O.: 1984 ‘Ultrastructural and Biochemical Nuclear Aspects of Eukaryote Classification: Independant Evolution of the Dinoflagellates as a Sister Group of the Actual Eukaryotes?’, Origins of Life 13 (3) (this issue).
Karsenti, E., Guilbert, B., Bornens, M., and Avrameas, S.: 1977, Proc. Natl. Acad. Sci. U.S.A. 74, 3997–4001.
Little, M., Luduena, R. F., Keenan, R., and Asnes, C. F.: 1982, ‘Tubulin Evolution: Two Major Types of α-tubulin’, J. Mol. Evol. 19, 80–86.
Little, M., Luduena, R. F., Langford, G. M., Asnes, C. F., and Farell, K.: 1981, ‘Comparison of Proteolytic Cleavage Patterns of α-tubulins and β-tubulins from Taxomomically Distant Species’, J. Mol. Biol. 149, 95–107.
Osborn, M. and Weber, K.: 1982, ‘Immunofluorescence and Immunocytochemical Procedures with Affinity Purified Antibodies: Tubulin-Containing Structures’, in L. Wilson (ed.), Methods in Cell Biology, Acad. Press, New York, 24, 97–132.
Taylor, F. J. R.: 1978, ‘Problems in the Development of an Explicit Hypothetical Phylogeny of the Lower Eucaryotes’, BioSystems 10, 67–89.
Thomashow, L. S., Milhausen, M., Rutter, W. J., and Agabian, N.: 1983, ‘Tubulin Genes Are Tandemly Linked and Clustered in the Genome of Trypanosoma brucei’, Cell 32, 35–43.
Wilde, C. D., Crowther, C. E., Cripe, T. P., Gwo-Shu, M., and Cowan, N. J.: 1982, ‘Evidence that a Human β-tubulin Pseudogene is Derived from Its Corresponding mRNA, Nature 297, 83–84.
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© 1984 D. Reidel Publishing Company
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Adoutte, A., Claisse, M., Cance, J. (1984). Tubulin Evolution: An Electrophoretic and Immunological Analysis. In: Margulis, L., Soyer-Gobillard, MO., Corliss, J. (eds) Evolutionary Protistology. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6398-6_2
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DOI: https://doi.org/10.1007/978-94-009-6398-6_2
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