Summary
The nodulation and the morphology and physiology of the nodules were studied on Datisca cannabina, a perennial herb from northern Pakistan and Alnus nitida, a nodulated tree in the same locality. Both species bear coralloid clusters of actinorhizal nodules. The main free amino acid in D. cannabina nodules was arginine while the predominant free amino acid in A. nitida nodules was citrulline. The infectivity of crushed nodules of both types of plants on their respective host was about 106 infective particles per gram of nodule fresh wt. In cross-inoculation experiments crushed nodule inoculum from A. nitida failed to induce nodulation on D. cannabina seedlings but the crushed nodule inoculum from D. cannabina caused low nodulation on seedlings of A. nitida (103 infective particles. g nodule fresh wt.).
The activity of nitrogenase, hydrogenase and respiration (O2 uptake) were measured in detached nodules, nodule homogenates and the 20 μm residue and 20 μm filtrate preparations from the nodules of both species. Both species showed similar patterns of activities except that only the nodule homogenate and 20 μm residue preparations from D. cannabina showed pronounced enhancement of the O2 uptake by succinate which was further stimulated by ADP. This has in part been explained by the presence of mitochondria in close connection with the endophyte.
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References
Abrams L 1952 An illustrated Flora of the Pacific States Washington, Oregon and California. 3, 143 Family 101, Stanford Univ. Press.
Akkermans A D L 1971 Nitrogen fixation and nodulation of Alnus and Hippophae under natural conditions. Thesis University Leiden, The Netherlands.
Akkermans A D L and Dijk C van 1981 Non-leguminous root-nodule symbioses with actinomycetes and Rhizobium. In Nitrogen Fixation Vol 1, Ecology, Ed. W J Broughton, Oxford University Press, Oxford, pp 57–103.
Akkermans A D L and Houwers A 1979 Symbiotic nitrogen fixers available in temperate forestry. In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry, Corvallis, Oregon USA, pp 23–35.
Akkermans A D L, Huss-Dannell K and Roelofsen W 1981 Enzymes of the citric acid cycle and the malate-aspartate shuttle in the N2-fixing endophyte of Alnus glutinosa. Physiol. Plant. 53, 289–294.
Akkermans A D L, Roelofsen W, Blom J, Huss-Danell K and Harkink R 1983 Utilization of carbon and nitrogen compounds by Frankia in synthetic media and in root nodules of Alnus glutinosa, Hippophaé rhamnoides and Datisca cannabina. Can. J. Bot. 61, 2793–2800.
Aseeva K B, Evstigneeva Z G and Kretovich V L 1966 Dokl. Akad. Nauk. SSR. 169, 463.
Blom J, Roelofsen W and Akkermans A D L 1981 Assimilation of nitrogen in root nodules of alder (Alnusglutinosa). New Phytol. 89, 321–326.
Bond G 1958 Symbiotic nitrogen fixation by non-legumes. In Nutrition of the legumes. Ed. E G Hallsworth, Butterworth and Co., London, pp 216–231.
Calvert H E, Chaudhary A H and Lalonde M 1979 Structure of an unusual root nodule symbiosis in a non-leguminous herbaceous dicotyledon. In Symbiotic Nitrogen fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry, Corvallis, Oregon, USA, pp 474–475.
Chaudhary A H 1975 Annual Second Research Report. PL-480 Research Project, Pk-FS32 (Grant No. FG-Pa-231), Department of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan, p 55.
Chaudhary A H 1978 The discovery of root nodules on new species of non-leguminous angiosperms from Pakistan and their significance. In Limitations and Potentials for Biological Nitrogen Fixation in the Tropics. Eds. J Dobereiner, R H Burris and A Hollaender, Plenum Press, New York and London, p 359.
Chaudhary A H 1979 Nitrogen-fixing root nodules in Datisca cannabina L. Plant and Soi 151, 163–165.
Chaudhary A H, Khokhar S N, Zafar Y and Hafeez F 1981 Actinomycetous root nodules in angiosperms of Pakistan. Plant and Soil 60, 341–348.
Davidson C 1973 An anatomical and morphological study of Datiscaceae. Aliso 8 (no 1), 49–110.
Davidson C 1976 Anatomy of xylem and phloem of the Datiscaceae. In Contributions in Science no 280, Natural History Museum of Los Angeles County.
Dijk C van and Merkus E 1976 A microscopic study of the development of a spore-like stage in the life cycle of the root-nodule endophyte of Alnus glutinosa (L.) Gaertn. New Phytol. 77, 73–91.
Hafeez F, Akkermans A D L and Chaudhary A H 1984 Observations on the ultrastructure of Frankia sp. in root nodules of Datisca cannabina L. Plant and Soil In press.
Hafeez F, Akkermans A D L and Chaudhary A H 1982 Some studies on the respiratory metabolism of the endophyte from the root nodules of Datisca cannabina and Alnus nitida. Pak. J. Bot. 14, 35.
Hegnauer R 1966 Chemotaxonomie der Pflanzen. 4, 11–12. Birkhauser Verlag Basel.
Hooker J D 1897 The Flora of British India. 2, 656–657. L. Reeve and Co Covent Garden London.
Humphries E C 1956 Determination of total nitrogen. In Modern Methods of Plant Analysis. Eds. K Peach and M V Tracey, Springer Verlag Berlin. Gottingen-Heidelberg, 1, pp 479–481.
Leaf G, Gardner C and Bond G 1958 Observations on the composition and metabolism of the nitrogen-fixing root nodules of Alnus. J. Exp. Bot. 9, 320–331.
Mason H L 1957 A Flora of the Marshes of California. Univ. Calif. Press Berkeley and Los Angeles, p 593.
Metcalfe C R and Chalk L 1957 Anatomy of Dicotyledons 1, 695–699. Oxford at the Clarendon Press.
Miettienen J K and Virtanen A I 1952 The free amino acids in the leaves, roots and root nodules of the alder (Alnus). Physiol. Plant. 5, 540–557.
Munz P A and Keck D D 1959 A California Flora. Univ. Calif. Press, p 177.
Noel A R A 1964 A staining and mounting combination of plant tissues. Stain Technol. 39,324.
Oremus P A I 1980 Occurrence and infective potential of the endophyte of Hippophaë rhamnoides L. ssp. rhamnoides in coastal sand-dune areas. Plant and Soil 56, 123–139.
Oremus P A I, Akkermans A D L and Nijholt L 1981 The effect of disintegration on the infective potential of the root-nodule endophyte of Hippophaë rhamnoides L. ssp. rhamnoides. Verhandelingen der Koninklijke Akademie van Wetenschappen, afd. Natuurkunde, 77, 45–50.
Roelofsen W and Akkermans A D L 1979 Uptake and evolution of H2 and reduction of C2H2 by root nodules and nodule homogenates of Alnus glutinosa. Plant and Soil 52, 571–578.
Siddiqi M A 1973 Datiscaceae. In Flora of West Pakistan Eds. E Nasir and S I Ali, 37, pp 1–3.
Severini G 1922 Sui tubercoli radicali di Datisca cannabina. Annali di Bot., Roma 15, 29–52.
Thomas 1961 Flora of the Santa Cruz Mountains of California. Stanford Univ. Press, p 243.
Wheeler C T and Bond G 1969 The amino acids of non-legume root nodules. Phytochemistry 9, 705–709.
Winship L J and Chaudhary A 1979 Nitrogen fixation by Datisca glomerata: a new addition to the list of actinorhizal diazotrophic plants. In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry, Corvallis, Oregon, USA, p 485.
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© 1984 Martinus Nijhoff/Dr W. Junk Publishers, The Hague
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Hafeez, F., Chaudhary, A.H., Akkermans, A.D.L. (1984). Physiological studies on N2-fixing root nodules of Datisca cannabina L. and Alnus nitida Endl. from Himalaya region in Pakistan. In: Akkermans, A.D.L., Baker, D., Huss-Danell, K., Tjepkema, J.D. (eds) Frankia Symbioses. Developments in Plant and Soil Sciences, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6158-6_12
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