Abstract
Restriction endonuclease analysis of virus DNA provides a suitable method to differentiate field isolates of Aujeszky’s disease virus (ADV). Inmost cases the differences that have been recognized in cleavage profiles of wild-type strains involved variations in the electrophoretic mobility of certain restriction fragments. Differences with respect to the presence or absence of cleavage sites were less frequently observed. Live-virus vaccine strains of ADV demonstrated unique DNA fragment patterns which were clearly different from one another and from those of wild-type virus isolates.
Restriction endonuclease analysis appeared to be a highly effective tool for (1) the identification of live-virus vaccine and wild-type virus in field cases of AD; (2) the demonstration of structural changes within the virus DNA during attenuation of virulent virus in tissue culture; (3) the study of persistence and spread of ADV within a pig herd and to trace the origin of infection; (4) the identification of the virus(es) excreted after corticosteroid treatment of latently infected pigs.
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© 1984 ECSC, EEC, EAEC, Brussels-Luxembourg
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Gielkens, A.L.J., van Oirschot, J.T., van Schie, F.W., Toma, B. (1984). Restriction Endonuclease Analysis of Aujeszky’s Disease Virus DNA. In: McNulty, M.S., McFerran, J.B. (eds) Recent Advances in Virus Diagnosis. Current Topics in Veterinary Medicine and Animal Science, vol 29. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6039-8_9
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DOI: https://doi.org/10.1007/978-94-009-6039-8_9
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