Viral Diagnosis by Immunofluorescence

  • P. S. Gardner
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 29)

Abstract

The main use of immunofluorescence in both human and veterinary virology should be for the rapid detection of antigen at site of lesion. For human disease WHO has strongly recommended immunofluorescence for rapid diagnosis of respiratory infection by detecting virus in nasopharyngeal secretions, and in the absence of an electron microscope for the investigation of skin and eye scrapings and brain biopsies. The principle of the technique and its methodology are briefly outlined and the use of conventional, egg-derived and monoclonal antibodies discussed. Some further advantages of the immunofluorescence technique are mentioned such as the investigation of outbreaks of respiratory disease in an animal house, the detection of antigen in secretions after infectivity of virus is lost, investigation of post-mortem specimens, control of cross-infection and diagnosis of infection at a distance from the virus laboratory. The major criticism of immunofluorescence is its labour intensiveness, which is balanced by its increased sensitivity and so far comparisons with alternative techniques such as ELISA have confirmed this increased sensitivity. Conjugated-staphylococcal protein A would appear to have no part in this technology as discussed and little information is available on the use of biotin-avidin as a detection system. Automation of reading fluorescence has been attempted by use of time-resolved fluoroimmunoassay, but it is too early to judge whether this technique is sufficiently practical, reproducible and economic for universal use.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Almeida, J.D. 1984. Electron microscopy. Recent Advances in Virus Diagnosis, (this volume).Google Scholar
  2. Downham, M.A.P.S., Elderkin, F.M., Platt, J.W., McQuillin, J. and Gardner, P.S. 1974. Rapid Virus Diagnosis in Paediatric Units by a Postal Service: Respiratory syncytial virus infection in Cumberland. Arch. Dis. in Childhood, 49, 467–471.CrossRefGoogle Scholar
  3. Downham, M.A.P.S., Gardner, P.S., McQuillin, J. and Ferris, J.A.J. 1975. Role of respiratory viruses in childhood mortality. Brit. Med. J., 1, 235–239.PubMedCrossRefGoogle Scholar
  4. Gardner, P.S., Court, S.D.M., Brocklebank, J.T., Downham, M.A.P.S. and Weightman, D. 1973. Virus Cross-infection in Paediatric Wards. Brit. Med. J., 2, 571–575.PubMedCrossRefGoogle Scholar
  5. Gardner, P.S. and McQuillin, J. 1978. The coating of R.S. virus infected cells in the respiratory tract by immunoglobulins. J. Med. Virol., 2, 77–87.CrossRefGoogle Scholar
  6. Gardner, P.S. and McQuillin, J. 1980. Rapid Virus Diagnosis. Application of immunofluorescence. 2nd Edition. (Butterworths, London).Google Scholar
  7. Gardner, P.S. and Kaye, S. 1982. Egg globulins in rapid virus diagnosis. J. Virol. Methods, 4, 257–262.PubMedCrossRefGoogle Scholar
  8. Halonen, P., Meurman, O., Lovgren, T., Hemmila, I. and Soini, E. 1983. Current topics in Microbiology and Immunology, Vol. 104. New Developments in Diagnostic Virology. Detection of viral antigens by time-resolved fluoroimmunoassay (Ed. Peter A. Bachmann). (Springer-Verlag, Berlin, Heidelberg), pp. 133–146.Google Scholar
  9. Memorandum. 1977. Laboratory techniques for rapid diagnosis of viral infections. Bulletin of the World Health Organization, 55, 33–37.Google Scholar
  10. Memorandum. 1978. Progress in the rapid diagnosis of viral infections. Bulletin of the World Health Organization, 56, 241–244.Google Scholar
  11. Richman, D.D. 1983. Current topics in Microbiology and Immunology, Vol. 104. New Developments in Diagnostic Virology. The use of staphylococcal Protein A in diagnostic virology. (Ed. Peter A. Bachmann). pp. 159–176.Google Scholar
  12. Russell, W.C., Patel, G., Precious, B., Sharp, I. and Gardner, P.S. 1981. Monoclonal antibodies against Adenovirus Type 5; Preparation and preliminary characterisation. J. Gen. Virol., 56, 393–408.PubMedCrossRefGoogle Scholar

Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1984

Authors and Affiliations

  • P. S. Gardner
    • 1
  1. 1.Division of Microbiological Reagents and Quality ControlCentral Public Health LaboratoryLondonUK

Personalised recommendations