Abstract
Chromatography involves the separation of the components of a mixture by virtue of differences in the equilibrium distribution (K) of the components between two phases: the mobile phase and the stationary phase. If C s and C m are the concentrations of a component in the stationary and mobile phases respectively, then:
Migration of component molecules may be assumed to occur only when the molecules are in the mobile phase. The rate of migration of a component is then inversely proportional to its distribution coefficient, so components with a high distribution in the stationary phase will move more slowly through the column and hence be separated from the components with a lower distribution in the stationary phase. Without this difference in distribution and, by inference, a differential rate of migration, no separation can be achieved.
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References
Giddings, J. C. (1965) Dynamics of Chromatography, Marcel Dekker, New York.
Halasz, I. (1971) in Modern Practice of Liquid Chromatography (ed. J. J. Kirkland), Wiley-Interscience, New York.
Huber, J. F. K. (1969) J. Chromatog. Sci., 7, 85.
Halasz, I., Endele, R. and Asshauer, J. (1975) J. Chromatog., 112, 37.
Grushka, E., Snyder, L. R. and Knox, J. H. (1975) J. Chromatog. Sci., 13, 25.
Snyder, L. R. (1972) J. Chromatog. Sci., 10, 369.
Done, J. H., Kennedy, G. J. and Knox, J. H. (1972) in Gas Chromatography (ed. S. G. Perry), Applied Science Publishers.
Deininger, G. and Halasz, I. (1970) in Advances in Chromatography (ed. A. Zlatkis), Preston Technical Abstracts Co., Chicago.
Scott, R. P. W. and Kucera, P. (1971) J. Chromatog. Sci, 9, 641.
Purnell, J. H. (1960) J. Chem. Soc., 1268.
Kozeny, J. S. B. (1927) Akad. Wiss. Wien, Abt. IIa 136, 271.
Carman, P. C. (1937) Trans. Inst. Chem. Eng. (London), 15, 150.
Halasz, I. and Heine, E. (1968) in Progress in Gas Chromatography (ed. J. H. Purnell), Interscience, New York.
Halasz, I. and Walkling, P. (1969) J. Chromatog. Sci., 7, 129.
Knox, J. H. and Parcher, J. F. (1969) Anal. Chem., 41, 1599.
Wheaton, R. M. and Bauman, W. C. (1953) Ann. New York Acad. Sci., 57, 159.
Giddings, J. C. (1967) Anal. Chem., 39, 1027.
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© 1982 R.J. Hamilton and P.A. Sewell
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Hamilton, R.J., Sewell, P.A. (1982). Chromatographic theory. In: Hamilton, R.J., Sewell, P.A. (eds) Introduction to high performance liquid chromatography. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5938-5_2
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DOI: https://doi.org/10.1007/978-94-009-5938-5_2
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