Obviously the main objective in the purification and isolation of viruses is the separation of the virus from the host tissues and cell organelles. With some Viruses this is easy, especially if the virus particle differs markedly in size from that of the normal cell contents. With other viruses purification may be much more difficult, if for instance the virus exists in very low concentration in the host. For this reason a number of viruses have never been isolated. With most plant and insect viruses it is possible to grind up or macerate the whole infected organism and clarify the crude extract by filtration, low-speed centrifugation and other methods. With the larger animals this is obviously impossible, though in certain cases, organs, such as the brain where the virus is known to be concentrated, can be removed and separately treated.
KeywordsVirus Particle Ammonium Sulphate Chinese Cabbage Virus Suspension Animal Virus
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