The quantitative assay of both plant and animal viruses is essentially a matter of counting cell lesions; in the case of plant viruses they are called ‘Local lesions’, and ‘Plaque assay’ in the case of animal viruses, the lesions consisting of small areas of cell destruction. For plaque assay cell culture is the essential factor, whereas special plants are used for the local lesion technique. In both cases it is necessary that the lesions remain localized for accurate counting; this is ensured by the choice of a plant which gives the right response and by restricting the spread of the lesions in the plaque by an overlay of molten agar (Dulbecco, 1952).
KeywordsVirus Particle Tobacco Mosaic Virus Plant Virus Local Lesion Plaque Assay
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