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Nitrogen Compounds

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Abstract

Although only 2% of the dry weight of plants consists of the element nitrogen, compared to 40% for carbon, there are still a very large number of different nitrogen-containing organic substances known in plants. Nitrogen is first available to the plant in the form of ammonia, produced either from nitrogen fixation in the root (symbiotically in legumes) or from enzvmic reduction of absorbed nitrate in shoot and leaf. Nitrogen first appears in organic form as glutamine, the key reactions being the transfer of ammonia to glutamic acid, catalysed by glutamine synthetase, followed by the transfer of nitrogen from glutamine to α-ketoglutarate, catalysed by glutamine α-ketoglutarate aminotransferase. These two enzymes operate together in what is often termed the GS-GOGAT pathway. The other amino acids are subsequently synthesized from the corresponding α-keto acids, the amino group being passed on from glutamic acid through the catalytic action of non-specific aminotransferases. Amino acids are also involved in the biosynthesis of practically all the other nitrogenous plant compounds, from the proteins (see Chapter 7) to the alkaloids, amines, cyanogenic glycosides, porphyrins, purines, pyrimidines and cytokinins.

Keywords

  • Aromatic Amine
  • Nitrogen Compound
  • Cyanogenic Glycoside
  • Pipecolic Acid
  • Bitter Almond

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

General references

  • Beevers, L. (1976) Nitrogen Metabolism in Plants, Edward Arnold, London.

    Google Scholar 

  • Brenner, M., Niederweiser, A. and Pataki, G. (1969) Amino acids and derivatives, in Thin Layer Chromatography (ed. E. Stahl), George Allen and Unwin, London, pp. 730–86.

    Google Scholar 

  • Clarke, E.G.C. (1970) The forensic chemistry of alkaloids, in The Alkaloids (ed. H.F. Manske) (Vol. XII), Academic Press, New York, pp. 514–90.

    Google Scholar 

  • Conn, E.E. and Butler, G.W. (1969) Biosynthesis of cvanogenic glycosides and other simple nitrogen compounds, in Perspectives in Phytochemistry (eds J.B. Harborne and T. Swain), Academic Press, London, pp. 47–74.

    Google Scholar 

  • Cordell, G.A. (1981) Introduction to Alkaloids, John Wiley, New York.

    Google Scholar 

  • Fowden, L. (1981) Non-protein amino acids, in Biochemistry of Plants (Secondary Plant Products, Vol. 7) (ed. E.E. Conn), Academic Press, New York, pp. 215–48.

    Google Scholar 

  • Hegnauer, R. (1967) Comparative phytochemistry of alkaloids, in Comparative Phytochemistry (ed. T. Swain), Academic Press, London, pp. 211–30.

    Google Scholar 

  • Hegnauer, R. (1977) Cyanogenic glycosides as systematic markers in Tracheophyta. Plant System. Evol., Suppl. 1, 191–210.

    Google Scholar 

  • Henry, T.A. (1949) The Plant Alkaloids, Churchill, London.

    Google Scholar 

  • Holden, M. (1976) Chlorophylls, in Chemistry and Biochemistry of Plant Pigments, 2nd edn, (ed. T.W. Goodwin) (Vol. 2), Academic Press, London, pp. 1–37.

    Google Scholar 

  • Horgan, R. (1978) Analytical procedures for cvtokinins. in Isolation of Plant Growth Substances (ed. J.R. Hillman), Cambridge University Press, Cambridge, pp. 97–114.

    Google Scholar 

  • Horgan, R. (1981) Modern methods for plant hormone analysis. Prog. Phytochem., 7, 137–70.

    Google Scholar 

  • Ikan, R. (1969) Natural Products: A Laboratory Guide, Academic Press. London, pp. 178–260.

    Google Scholar 

  • Jackson, A.H. (1976) Structure, properties and distribution of chlorophylls, in Chemistry and Biochemistry of Plant Pigments, 2nd edn (ed. T.W. Goodwin), Academic Press, London, pp. 1–63.

    Google Scholar 

  • Jones, D.A. (1972) Cyanogenic glycosides and their function, inPhytochemical Ecology (ed. J.B. Harborne), Academic Press, London, pp. 103–24.

    Google Scholar 

  • Kaldewey, H. (1969) Simple indole derivatives and plant growth regulators, in Thin Layer Chromatography (ed. E. Stahl), George Allen and Unwin, London, pp. 471–93.

    Google Scholar 

  • MacMillan, J. (ed.) (1980) Hormonal Regulation of Development, Molecular Aspects of Plant Hormones, Springer-Verlag, Berlin.

    Google Scholar 

  • Miflin, B.J. (ed.) (1981) Biochemistry of Plants (Amino Acids and Derivatives, Vol. 5), Academic Press, New York.

    Google Scholar 

  • Randerath, K. and Randerath, E. (1967) Thin layer methods for nucleic acid derivatives. in Methods in Enzymology (Vol. XII A), Academic Press, New York, pp. 323–47.

    Google Scholar 

  • Robinson, T. (1981) The Biochemistry of Alkaloids, 2nd edn. Springer-Verlag, Berlin.

    Google Scholar 

  • Rodrigo, R.G.A. (ed.) (1982) The Alkaloids (Chemistry and Physiology, Vol. 20), Academic Press, New York.

    Google Scholar 

  • Rosenthal, G.A. (1982) Plant non-protein Amino and Imino Acids, Academic Press, New York.

    Google Scholar 

  • Seiber, N. (1970) in Methods of Biochemical Analysis (ed. D. Glick), John Wiley and Sons, New York, pp. 259–337.

    Google Scholar 

  • Smith, T.A. (1981) Amines, in Biochemistry of Plants (Secondary Plant Products, Vol. 7) (ed. E.E. Conn), Academic Press, New York, pp. 249–68.

    Google Scholar 

  • Venkstern, TV. and Baer, A.A. (1966) Absorption Spectra of Minor Bases, their Nucleosides, Nucleotides and of Selected Oligoribonucleotides, Plenum Press, New York.

    Google Scholar 

  • Vernon, L.P. and Seeley, G.R. (1966) The Chlorophylls, Academic Press, New York.

    Google Scholar 

Supplementary references

  • Bacon, M.F. (1965) J. Chromatog., 17, 322.

    CrossRef  Google Scholar 

  • Baker, W., Harborne, J.B. and Ollis, W.D. (1952) J. Chem. Soc., 3215.

    Google Scholar 

  • Beaven, G.H., Holiday, E.R. and Johnson, E.A. (1955) in The Nucleic Acids (ed. E. Chargaff and J.N. Davidson) (Vol. 1), Academic Press, New York, pp. 493–533.

    Google Scholar 

  • Bell, E.A., Lackey, J.A. and Polhill, R.M. (1978) Biochem. System. Ecol., 6, 201.

    CrossRef  Google Scholar 

  • Bell, E.A. and Tirimanna, A.S.L. (1965) Biochem. J., 97, 104.

    Google Scholar 

  • Bieleski, R.L. and Turner, N.A. (1966) Analyt. Biochem., 17, 278.

    CrossRef  Google Scholar 

  • Bissett, F.H., Clapp, R.C., Coburn, R.A., Ettlinger, M.G. and Long, L. (1969) Phyto-chemistry, 8, 2235.

    Google Scholar 

  • Blackburn, S. (1965) Meth. Biochem. Anal., 13, 1.

    CrossRef  Google Scholar 

  • Booth, J. and Bovland, E. (1964) Biochem. J., 91, 364.

    Google Scholar 

  • Burnett, D. and Audus, LJ. (1964) Phytochemistry. 3, 395.

    CrossRef  Google Scholar 

  • Conn, E.E. (1979) in Herbivores, their Interaction with Secondary Plant Metabolites (eds G.A. Rosenthal and D.H. Janzen), Academic Press. New York, pp. 387–112.

    Google Scholar 

  • Daday, H. (1954) Heredity, 8, 61, 377.

    CrossRef  Google Scholar 

  • Dolphin, D. (1983) in Chromatography, Fundamentals and Applications, Part B (ed. E. Heftmann), Elsevier, Amsterdam, pp. 377–406.

    CrossRef  Google Scholar 

  • Dunnill, P.M. and Fowden, L. (1965) Phytochemistry. 4, 933.

    CrossRef  Google Scholar 

  • Ek, M., Ljungquist, P.O. and Stenstrom, E. (1983) New Phytologist, 94, 401.

    CrossRef  Google Scholar 

  • Fawcett, C.H., Wain, R.L. and Wightman, F. (1960) Proc. R. Soc. Ser. B. 152, 231.

    CrossRef  Google Scholar 

  • Feigl, F. and Anger, V.A. (1966) Analyst, 91, 282.

    CrossRef  Google Scholar 

  • Fellows, L.E. and Bell, E.A. (1970) Phytochemistry, 9, 2389.

    CrossRef  Google Scholar 

  • Grant, W.F. and Sidhu, B.S. (1967) Can. J. Bot., 45, 639.

    CrossRef  Google Scholar 

  • Hanes, C., Harris, C.K. and Moscarella, M.A. (1961) Can. J. Biochem. Physiol., 39, 163, 439.

    Google Scholar 

  • Horwitz, W. (ed.) (1965) Official Methods of Analysis of the Association of Official Agricultural Chemists, 10th edn, AOAC, Washington DC, U.S.A., p. 341.

    Google Scholar 

  • Hultin, E. (1966) Acta Chem. Scand., 20, 1588.

    CrossRef  Google Scholar 

  • Hultin, E. and Torsell, K. (1965) Phytochemistry, 4, 425.

    CrossRef  Google Scholar 

  • Ilert, H.I. and Hartmann, T. (1972) J. Chromatog., 71, 119.

    CrossRef  Google Scholar 

  • Irvine, W.J. and Saxby, M.J. (1969) Phytochemistry, 8, 473.

    CrossRef  Google Scholar 

  • Kamienski, E.S. van (1959) in Papier Chromatographie in der Botanik (ed. H.F. Linskens), Springer-Verlag, Berlin.

    Google Scholar 

  • Kipps, A. (1972) Ph.D. Thesis, University of Durham.

    Google Scholar 

  • Kofod, H. and Eyjolfsson, R. (1969) Phytochemistry, 8, 1509.

    CrossRef  Google Scholar 

  • Kuster, T. and Niederwieser, A. (1983) in Chromatography, Fundamentals and Applications, Part B (ed. E. Heftmann), Elsevier, Amsterdam, pp. 1–51.

    CrossRef  Google Scholar 

  • Lawler, L.J. and Slavtor, M. (1969) Phytochemistry, 8, 1959.

    CrossRef  Google Scholar 

  • Lederer, E. and Lederer, M. (1957) Chromatography, A Review of Principles and Applications, Elsevier, Amsterdam.

    Google Scholar 

  • Letham, D.S. (1963) Life Sci., 8, 569.

    CrossRef  Google Scholar 

  • Lichtenthaler, H.K. and Wellburn, A.R. (1983) Biochem. Soc. Trans., 11, 591.

    Google Scholar 

  • Lüning, B. (1967) Phytochemistry, 6, 852.

    CrossRef  Google Scholar 

  • McDougall, J. and Hillman, J.R. (1978) in Isolation of Plant Growth Substances (ed. J.R. Hillman), Cambridge University Press, Cambridge, pp. 1–26.

    Google Scholar 

  • Macek, K. (1967) in Chromatography (ed. E. Heftmann), Reinhold, New York, pp. 606–26.

    Google Scholar 

  • Maher, E.P. and Hughes, M.A. (1971) Phytochemistry, 10, 3005.

    CrossRef  Google Scholar 

  • Mangold, H.K. (1969) in Thin Layer Chromatography (ed. E. Stahl), George Allen and Unwin, London, pp. 786–804.

    Google Scholar 

  • Markham, R. (1955) in Modern Methods of Plant Analysis (eds K. Paech and M.V. Tracey) (Vol. IV). Springer Verlag, Berlin, pp. 246–304.

    Google Scholar 

  • Miller, C.O., Skoog, F., Okumura, F.S., Von Saltza, M.H. and Strong, F.M. (1956) J Am. Chem. Soc., 78, 1375.

    CrossRef  Google Scholar 

  • Mischke, C.F. and Wickstrom, E. (1980) Analyt. Biochem., 105, 181.

    CrossRef  Google Scholar 

  • Mollan, R.C., Harmey, M.A. and Donnelly, D.M.X. (1973) Phytochemistry, 12, 447.

    CrossRef  Google Scholar 

  • Moore, S. and Stein, W.H. (1956) Adv. Protein Chem., 11, 191.

    CrossRef  Google Scholar 

  • O’Carra, P. and O’Eocha, C. (1976) in Chemistry and Biochemistry of Plant Pigments. 2nd edn (ed. T.W. Goodwin) (Vol. 1), Academic Press, London, pp. 328–77.

    Google Scholar 

  • Phillipson, J.D. (1982) Phytochemistry, 21, 2441.

    CrossRef  Google Scholar 

  • Pusev, J.G. (1963) in Teaching Genetics (eds C.D. Darlington and DA. Bradshaw). Oliver and Boyd, Edinburgh, pp. 99–104.

    Google Scholar 

  • Santavy, F. (1969) in Thin Layer Chromatography (ed. E. Stahl), George Allen and Unwin. London, pp. 421–70.

    Google Scholar 

  • Schneider, E.A., Gibson, R.A. and Wightman, F. (1972) J. Exp. Bot., 23, 152.

    CrossRef  Google Scholar 

  • Seigier, D.S. (1977) Prog. Phytochem., 4, 83.

    Google Scholar 

  • Sharpies, D. and Stoker, J.R. (1969) Phytochemistry, 8, 597.

    CrossRef  Google Scholar 

  • Smith, T.A. (1970) Phytochemistry, 9, 1479.

    CrossRef  Google Scholar 

  • Smith, T.A. (1972) Private communication.

    Google Scholar 

  • Stahl, E. and Schorn, P.J. (1969) in Thin Layer Chromatography (ed. E. Stahl). George Allen and Unwin. London, pp. 494–505.

    Google Scholar 

  • Stowe, B., Vendrell, M. and Epstein, E. (1968) in Biochemistry and Physiology of Plant Growth Substances (eds F. Wightman and G. Setterfield), Runge Press. Ottawa, pp. 173–82.

    Google Scholar 

  • Stransky, H. (1978) Z. Naturforsch., 33c, 836.

    Google Scholar 

  • Taber, W.A., Vining, L.C. and Heacock, R.A. (1963) Phytochemistry, 2, 65.

    CrossRef  Google Scholar 

  • Waldi, D., Schnackerz, K. and Munter, F. (1961) J. Chromat., 6, 61.

    CrossRef  Google Scholar 

  • Weiler, E.W. (1983) Biochem. Soc. Trans., 11, 485.

    Google Scholar 

  • Wightman, F. and Cohen, D. (1968) in Biochemistry and Physiology of Plant Growth Substances (eds F. Wightman and G. Setterfield), Runge Press, Ottawa, pp. 273–88.

    Google Scholar 

  • Witham, F.H., Blavdes, D.F. and Devlin, R.M. (1971) Experiments in Plant Physiology, Van Nostrand, New York, pp. 44–6.

    Google Scholar 

  • Wood, T. (1966) J. Sci, Food Agr., 17, 85.

    CrossRef  Google Scholar 

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© 1984 J. B. Harborne

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Harborne, J.B. (1984). Nitrogen Compounds. In: Phytochemical Methods. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5570-7_5

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