Abstract
Thyrotropin (TSH) is a pituitary glycoprotein hormone whose primary role is to regulate thyroid cell function (Dumont, 1971; Field, 1980; Robbins et al., 1980; Kohn et al., 1983). The interaction of TSH with a specific receptor on the thyroid cell surface induces changes in adenylate cylcase activity which result in the following tissue responses: enhanced iodide uptake, thyroglobulin biosynthesis, iodination of thyroglobulin, degradation of iodinated thyroglobulin to form thyroid hormone, and the release of thyroid hormone (T3 and T4) into the bloodstream. Although changes in all of the above activities have been and can be used to define receptor function, definition of the structure of the TSH receptor on a molecular level, as with all other receptors, has required the identification of specific membrane components using binding studies and 125I-labeled hormone (Kohn, 1978; Kohn and Shifrin, 1982; Kohn et al., 1983).
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Abbreviations
- TSH:
-
Thyrotropin (thyroid-stimulating hormone)
- hCG:
-
Human chorionic gonadotropin
- LH:
-
Luteinizing hormone
- FSH:
-
Follicle-stimulating hormone
- LIS:
-
Lithium diiodosalicylate
- FRTL-5 cells:
-
Functioning rat thyroid cells derived from normal Fisher rat thyroid glands
- GM1 :
-
Galactosyl-N-acetylgalactosaminyl-[N-acetylneuraminyl]galactosyl-glucosylceramide
- GM2 :
-
N-Acetylgalactosaminyl-[N-acetylneuraminyl]galactosylglucosylceramide
- GM3 :
-
N-Acetylneuraminylgalactosylglucosylceramide
- GDla :
-
N-Acetylneuraminylgalactosyl-N-acetylgalactosaminyl-N-acetylneuraminyl]galactosylglucosylceramide
- GDlb :
-
Galactosyl-N-acetylgalactosaminyl-[N-acerylneuraminyl-N-acetylneuraminyl]galactosylglucosylceramide
- GT1 :
-
N-Acetylneuraminylgalactosyl-N-acetylgalactosaminyl-[N-acetylneuraminyl-N-acetylneuraminyl]galactosylglucosylceramide
- T3 :
-
Tri-iodothyronine
- T4 :
-
Thyroxine
- CBA:
-
Cytochemical bioassay
- TSAb:
-
Thyroid-stimulating antibody
- FCCP:
-
Carbonyl cyanide p-trifluoromethoxyphenylhydrazone
References
Adams, D.D. (1981), in Vitam. Horm., 38 119–203.
Aloj, S.M., Kohn, L.D., Lee, G. and Meldolesi, M.F. (1977), Biochem. Biophys. Res. Commun., 74 1053–1059.
Aloj, S.M., Lee, G., Grollman, E.F., Beguinot, F., Consiglio, E. and Kohn, L.D. (1979), J. Biol. Chem., 254 9040–9049.
Ambesi-Impiombato, F.S., Park, L.A.M. and Coon, H.G. (1980), Proc. Natl. Acad. Sci. U.S.A., 77 3455–3459.
Beckner, S.K., Brady, R.O. and Fishman, P.H. (1981), Proc. Natl. Acad. Sci. U.S.A., 78 4848–4853.
Bennett, V. and Cuatrecasas, P. (1977), in The Specificity and Action of Animal, Bacterial, and Plant Toxins (Cuatrecasas, P. and Greaves, M.F, eds), Chapman and Hall, London, pp. 3–66.
Bitensky, L., Alaghband-Zadeh, J. and Chayen, J. (1974), Clin. Endocrinol. (Oxf), 3 363–374.
Chayen, J., Bitensky, L. and Butcher, R.G. (1973), Practical Histochemistry, Wiley, London.
Chayen, J., Daly, J.R., Loveridge, N. and Bitensky, L. (1976), Recent Prog. Horm. Res., 32 33–79.
Davies, T.F., Yeo, P.P.B., Evered, D.C., Clark, F., Smith, R.R. and Hall, R. (1977), Lancet, i 1181–1185.
De Wolf, M., Vitti, P., Ambesi-Impiombato, F.S. and Kohn, L.D. (1981), J. Biol. Chem., 256 12287–12296.
Doniach, D. (1975), Clin. Endocrinol. Metab., 4 267–285.
Dumont, J.E. (1971), Vitam. Horm., 29 287–412.
Ealey, P.A., Marshall, N.J. and Ekins, R.P. (1981), J. Clin. Endocrinol. Metab., 52 483–487.
Ealey, P.A., Kohn, L.D., Ekins, R.P. and Marshall, N.J. (1984a), J. Clin. Endocrinol. Metab., (in press).
Ealey, P.A., Valente, W., Kohn, L.D., Ekins, R.P. and Marshall, N.J. (1984b), Endocrinology, (in press).
Field, J.B. (1980), in The Thyroid 4th ed.(Werner, S.C. and Ingbar, S.H., eds). Harper and Row, Hagerstown, Maryland, pp. 185–195.
Friedman, R.M., Lee, G., Shifrin, S., Ambesi-Impiombato, S., Epstein. D., Jacobsen, H. and Kohn, L.D. (1982), J. Interferon Res., 2 387–400.
Gill, D.M. (1977), Adv. Cyclic Nucleotide Res., 8 85–118.
Grollman, E.F., Lee, G., Ramos, S., Layo, P.S., Kaback, H.R., Friedman, R.M. and Kohn, L.D. (1978), Cancer Res, 38 4172–4185.
Holmgren, J. (1978), in Bacterial Toxin and Cell Membranes (Jeljaczewicz, Z. and Wadstrom, E., eds). Academic Press, London, pp. 333–366.
Kohn, L.D. (1978), in Receptors and Recognition (Cuatrecasas, P. and Greaves, M.F., eds), Chapman and Hall, London. Vol. 5, Series A, pp. 133–212.
Kohn, L.D. and Shifrin, S. (1982). in Horizons in Biochemistry and Biophysics, Hormone Receptors (Kohn, L.D., ed.), J. Wiley and Sons, New York, Vol. 6, pp. 1–42.
Kohn, L.D., Consiglio, E., De Wolf, M.J.S., Grollman, E.F., Ledley, F.D., Lee, G. and Morris, N.P. (1980), in Structure and Function of Gangliosides (Svennerholm, L., Mandel, P., Dreyfus, H. and Urban, P.F.. eds). Plenum Press. New York, pp. 487–504.
Kohn, L.D., Consiglio, E., Aloj, S.M.. Beguinot, F., De Wolf, M.J.S., Yavin, E., Yavin, Z., Meldolesi, M.F., Shifrin, S., Gill, D.L., Vitti, P., Lee, G., Valente, W.A. and Grollman, E.F. (1981), in International Cell Biology 1980–1981 (Schweiger, A.G., ed.), Lange and Springer, Berlin, pp. 696–706.
Kohn, L.D., Aloj, S.M., Beguinot, F., Vitti, P., Yavin, E., Yavin, Z., Laccetti, P., Grollman, E.F. and Valente, W.A. (1982a), in Membranes and Genetic Diseases (Shepard, J., ed.), Alan R. Liss, New York, Vol. 97, pp. 55–83.
Kohn, L.D., Valente, W.A., Laccetti, P., Cohen, J.L., Aloj, S.M. and Grollman, E.F. (1982b), Life Sci, 32 15–30.
Kohn, L.D., Aloj, S.M., Shifrin, S., Valente, W.A., Weiss, S.J., Vitti, P., Laccetti, P., Cohen, J.L., Rotella, C.M. and Grollman, E.F. (1983), in Receptors for Polypeptide Hormones (Posner, B.T., ed.). Marcel Dekker, New York, (in press).
Laccetti, P. Grollman, E.F., Aloj, S.M. and Kohn, L.D. (1983), Biochem. Biophys. Res. Commun., 110 772–778.
Laccetti, P., Vitti, P., Rotella, C.M., Grollman, E.F., Valente, W.A., Cohen, J.L., Aloj, S.M. and Kohn, L.D. (1984). Endocrinology, (in press).
Mandato, E.M., Meldolesi, F. and Macchia, V. (1975), Cancer Res, 35 3089–3093.
KcKenzie, J.M. (1958), Endocrinology, 63 372–384.
McKenzie, J.M. and Zakarija, J. (1976), J. Clin. Endocrinol. Metab., 42 778–781.
Meldolesi, M.F., Fishman, P.H., Aloj, S.M., Kohn, L.D. and Brady, R.O. (1976), Proc. Natl. Acad. Sci. U.S.A., 73 4060–4064.
Moss, J. and Vaughan, M. (1979), Annu. Rev. Biochem., 48 581–600.
Pekonen, F. and Weintraub, B.D. (1980), J. Biol. Chem., 255 8121–8127.
Pinchera, A., Fenzi, G.F., Macchia, E., Vitti, P., Monzani, F. and Kohn, L.D. (1982), Ann. Endocrinol. (Paris), 43 520–533.
Robbins, J., Rall, J.E. and Gorden, P. (1980), in Metabolic Control and Disease (Bondy, P.K. and Rosenberg, L.E., eds), W.B. Saunders, Philadelphia, pp. 1325–1426.
Rotella, C.M., Tramantano, D., Kohn, L.D., Aloj, S.M., Ambesi-Impiombato, F.S. and Kohn, L.D. (1984), Endocrinology, (in press).
Sluszkiewicz, E. and Pawlikowski, M. (1980), Endocrinol,Exp, 14 227–235.
Tate, R.L., Schwartz, H.I., Holmes, J.M., Kohn, L.D. and Winand, R.J. (1975), J. Biol. Chem., 250 6509–6515.
Valente, W.A., Vitti, P., Kohn, L.D., Brandi, M.L., Rotella, C.M., Toccafondi, R., Tramontano, D., Aloj, S.M. and Ambesi-Impiombato, F.S. (1982a), Endocrinology, 112 71–79.
Valente, W.A., Yavin, Z., Yavin, E., Grollman, E.F., Schneider, M.D., Rotella, C., Zonefrati, R., Toccafondi, R.S. and Kohn, L.D. (1982b), J. Endocrinol. Invest., 5 293–301.
Valente, W.A., Vitti, P., Yavin, Z., Yavin, E., Rotella, C.M., Grollman, E.F., Toccafondi, R.S. and Kohn, L.D. (1982c), Proc. Natl. Acad. Sci. U.S.A., 79 6680–6684.
Valente, W.A., Vitti, P., Rotella, C.M., Vaughan, M.M., Aloj, S.M., Grollman, E.F., Ambesi-Impiombato, F.A. and Kohn, L.D. (1983), N. Engl. J. Med., 309 1038–1044.
Valente, W.A., Yavin, E., Yavin, Z. and Kohn, L.D. (1984), Endocrinology, (in press).
Vitti, P., De Wolf, M.J.S., Acquaviva, A.M., Epstein, M. and Kohn, L.D. (1982), Proc. Natl. Acad. Sci. U.S.A., 79 1525–1529.
Vitti, P., Rotella, C.M., Valente, W.A., Cohen. J.L., Aloj, S.M., Laccetti, P., Ambesi-Impiombato, F.S., Grollman, E.F., Pinchera, A., Toccafondi, R. and Kohn, L.D. (1983), J. Clin. Endocrinol. Metab., 57 782–791.
Weiss, S., Philip, N.J., Ambesi-Impiombato, F.S. and Grollman, E.F. (1984), Endocriniology, (in press).
Winand, R.J. and Kohn, L.D. (1975), J. Biol. Chem., 250 6534–6540.
Yavin, E., Yavin, Z., Schneider, M.D. and Kohn, L.D. (1981a), Proc. Natl. Acad. Sci. U.S.A., 78 3180–3184.
Yavin, E., Yavin, Z., Schneider, M.D. and Kohn, L.D. (1981b), in Monoclonal Antibodies in Endocrine Research (Fellows, R.E. and Eisenbarth, G., eds), Raven Press, New York, pp. 53–67.
Yavin, E., Yavin, Z., Schneider, M.D. and Kohn, L.D. (1981c), in Monoclonal Antibodies to Neural Antigens (McKay, R., Raff, M. and Reichardt, L., eds), Cold Spring Harbor Laboratories, New York, pp. 141–152.
Zakarija, J. and McKenzie, J.M. (1980). in Autoimmune Aspects of Endocrine Disorders (Pinchera, A., Doniach, D., Fenzi, G.F. and Baschieri, L., eds), Academic Press, New York, pp. 83–90.
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Kohn, L.D., Tombaccini, D., De Luca, M.L., Bifulco, M., Grollman, E.F., Valente, W.A. (1984). Monoclonal Antibodies and the Thyrotropin Receptor. In: Greaves, M.F. (eds) Monoclonal Antibodies to Receptors. Receptors and Recognition, vol 17. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5566-0_9
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