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Interrelationship Among Aging, Cancer and Differentiation pp 267–275Cite as

Monoclonal Antibodies Directed Against Alkyl-Deoxynucleosides and Cell Type- and Differentiation Stage-Specific Cell Surface Determinants in the Study of Ethylnitrosourea-Induced Carcinogenesis in the Developing Rat Brain

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Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 18))

Abstract

In this report we describe some properties and applications of two different groups of monoclonal antibodies (Mab) produced in our laboratory for the analysis of molecular and cellular aspects of the process of carcinogenesis induced by N-ethyl-N-nitrosourea (EtNU) in the developing rat brain, Mab of the first group are directed against defined alkylation products in the DNA of cells exposed to alkylating N-nitroso compounds. In conjunction with sensitive immunoanalytical methods, these Mab are used for quantitation of alkyl-deoxynucleosides in the DNA of tissues and individual cells, for studies of DNA repair, and for analyses of the frequency and distribution of specific alkyldeoxynucleosides in the DNA of different chromatin fractions and in individual DNA molecules. Mab of the second group recognize and bind to cell type- and developmental stage-specific cell surface determinants (CSD) of rat brain cells (BC). These Mab are presently applied for the separation by fluorescence-activated cell sorting (and subsequent in vitro culture) of distinct BC subpopulaticns previously exposed to EtNU in vivo, as well as for comparative CSD analyses of defined subpopulations of BC and their malignant counterparts.

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Author information

Authors and Affiliations

  1. Institut für Zellbiologie (Tumorforschung), Universität Essen (GH), Hufelandstrasse 55, D-4300, Essen 1, Federal Republic of Germany

    M. F. Rajewsky, J. Adamkiewicz, N. Huh, A. Kindler-Röhrborn, U. Langenberg, R. Minwegen & P. Nehls

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  1. M. F. Rajewsky
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  2. J. Adamkiewicz
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  3. N. Huh
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  4. A. Kindler-Röhrborn
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  5. U. Langenberg
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  6. R. Minwegen
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  7. P. Nehls
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Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Paris, France

    Bernard Pullman

  2. Division of Biophysics, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland, USA

    Paul O. P. Ts’o

  3. National Institute on Aging, National Institutes of Health, Bethesda, Maryland, USA

    Edmond L. Schneider

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© 1985 D. Reidel Publishing Company, Dordrecht, Holland

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Rajewsky, M.F. et al. (1985). Monoclonal Antibodies Directed Against Alkyl-Deoxynucleosides and Cell Type- and Differentiation Stage-Specific Cell Surface Determinants in the Study of Ethylnitrosourea-Induced Carcinogenesis in the Developing Rat Brain. In: Pullman, B., Ts’o, P.O.P., Schneider, E.L. (eds) Interrelationship Among Aging, Cancer and Differentiation. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 18. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5466-3_26

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  • DOI: https://doi.org/10.1007/978-94-009-5466-3_26

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8913-5

  • Online ISBN: 978-94-009-5466-3

  • eBook Packages: Springer Book Archive

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