Conifer Protoplasts

Part of the Forestry Sciences book series (FOSC, volume 24-26)


Genetic modification of cells by somatic hybridization or by the introduction of organelles (chloroplasts, mitochondria) and DNA or RNA molecules (via liposomes) has a bright future, thanks to modern protoplast technology. However, it may be rather Utopian as yet to wish to genetically modify conifers by isolation and culture of protoplasts because this group of trees is notoriously difficult to manipulate in vitro. Nevertheless, there are at least three good reasons to attempt culture of conifer protoplasts. First, the protoplast system permits reprogramming of the nucleus and cytoplasm, which could facilitate cellular rejuvenation and thus a return to the expression of totipotency. Second, it is convenient material for experimentation in genetic engineering of conifers. Third, cells are now available that have the capacity to form somatic embryos and these are logical sources for the production of protoplasts (cf. Tulecke and Durzan in this volume).


Somatic Embryo Mesophyll Protoplast Protoplast Isolation Pinus Pinaster Protoplast Culture 
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© Martinus Nijhoff Publishers, Dordrecht 1987

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