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Cold Storage of Tissue Cultures

Chapter
Part of the Forestry Sciences book series (FOSC, volume 24-26)

Abstract

Collections of seed and clonal material are traditional ways of storing genetic resources, while tissue cultures provide a complementary way. Freeze-preservation of cultured cells and meristems has become popular for long-term germplasm preservation (7,28,53). However, freeze-preservation requires the use of sophisticated techniques and expensive equipment, and often leads to tissue damage during freezing and thawing cycles. Another way of storing germplasm which does not require transfers is to limit growth by modification of the medium, e.g., by adding osmotically active substances. Cold storage of cultures at 0 to 15°C is a third method.

Keywords

Cold Storage Cold Acclimation Plant Tissue Culture Malus Domestica Forest Research Institute 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishers, Dordrecht 1987

Authors and Affiliations

  1. 1.Forest Research InstituteRotoruaNew Zealand

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