Cold Storage of Tissue Cultures

Part of the Forestry Sciences book series (FOSC, volume 24-26)


Collections of seed and clonal material are traditional ways of storing genetic resources, while tissue cultures provide a complementary way. Freeze-preservation of cultured cells and meristems has become popular for long-term germplasm preservation (7,28,53). However, freeze-preservation requires the use of sophisticated techniques and expensive equipment, and often leads to tissue damage during freezing and thawing cycles. Another way of storing germplasm which does not require transfers is to limit growth by modification of the medium, e.g., by adding osmotically active substances. Cold storage of cultures at 0 to 15°C is a third method.


Cold Storage Cold Acclimation Plant Tissue Culture Malus Domestica Forest Research Institute 
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  1. 1.
    AITKEN-CHRISTIE J 1984 Micropropagation of Pinus radiata. The Plant Propagator 30: 9–11Google Scholar
  2. 2.
    AITKEN-CHRISTIE J. J GLEED 1985 Uses for micropropaga-tion of juvenile radiata pine in New Zealand. In Proc Int Symp of Recent Advances in Forest Biotechnology, Traverse City, Michigan, pp 47–57Google Scholar
  3. 3.
    AITKEN-CHRISTIE J, KJ HORGAN, DR SMITH 1982 Micro-formering af skovtraeer. Ugeskrift for Jordbrug 19: 375–382Google Scholar
  4. 4.
    AITKEN-CHRISTIE J, C JONES, S BOND 1985 Wet and waxy shoots in radiata pine micropropagation. Acta Hort 166: 93–100Google Scholar
  5. 5.
    ANON 1982 Institutes working on tissue culture for genetic conservation. In L Withers, ed. International Board for Plant Genetic Resources Report. IBPGR Secretariat Rome, pp 64–69Google Scholar
  6. 6.
    ANON 1982 Les tailles d’ Eucalyptus. In Culture de Biomasse Ligneuse, Taillis à Courte Rotation. AFOCEL-Armef. Nangis, pp 96–145Google Scholar
  7. 7.
    BAJAJ YPS 1979 Establishment of germplasm banks through freeze-storage of plant tissue culture and their implications in agriculture. In WR Sharp, PO Larsen, EF Paddock. V Raghavan, eds. Plant Cell and Tissue Culture, Principles and Applications. Ohio State Univ Press, Columbus, pp 745–774Google Scholar
  8. 8.
    BANNIER LJ, P STEPONKUS 1976 Cold acclimation of Chrysanthemum callus cultures. J Amer Soc Hort Sci 101: 409–412Google Scholar
  9. 9.
    BARLASS M. KGM SKENE 1983 Long-term storage in grape in vitro Int Board Plant Genet Res Newslett 53: 19–21Google Scholar
  10. 10.
    BHOJWANI S, MK RADZAN 1983 Germplasm storage. In S Bhojwani, MK Radzan, authors. Plant Tissue Culture: Theory and Practice. Elsevier. Amsterdam, pp 373–385Google Scholar
  11. 11.
    BONGA JM 1981 Organogenesis in vitro of tissues from mature conifers. In Vitro 17: 511–518CrossRefGoogle Scholar
  12. 12.
    BOXUS P, P DRUART 1980 Micropropagation, an industrial method of producing quality plants true to type and at a reasonable price. In F Sala. B Parisi. R Cella, O Ciferri, eds. Plant Cell Cultures: Results and Perspectives. Elsevier/North-Holland Biomed Press, pp 265–269Google Scholar
  13. 13.
    BOXUS P, P DRUART, E BRASSEUR 1979 Tissue culture of fruit species. Report for 1979 of the State Centre for Agricultural Research. Gembloux, Belgium, pp 116–117Google Scholar
  14. 14.
    BROWN GN 1978 Protein synthesis mechanisms relative to cold hardiness. In PH Li, A Sakai, eds. Plant Cold Hardiness and Freezing Stress. Academic Press. New York, pp 153–163Google Scholar
  15. 15.
    CORNU D, C CHAIX 1981 Multiplication par culture in vitro de merisiers adultes (Prunus avjum). In Proc IUFRO Sect S2 01 5. Int Workshop “In Vitro” Cultivation for Tree Species, Fontainbleu, France, pp 71–79Google Scholar
  16. 16.
    DAMIANO C 1979 Cold storage of in vitro strawberry cultures and the resumption of multiplication. Ann Istit Sper Frutticolt 10: 53–58Google Scholar
  17. 17.
    DIX PJ, HE STREET 1976 Selection of plant cell lines with enhanced chilling resistance. Ann Bot 40: 903–910Google Scholar
  18. 18.
    FARNUM P. R TIMMIS, JL KULP 1983 Biotechnology of forest yield. Science 219: 694–702PubMedCrossRefGoogle Scholar
  19. 19.
    FRANCLET A, M BOULAY 1982 Micropropagation of frost resistant Eucalypt clones. Aust For Res 13: 83–89Google Scholar
  20. 20.
    FEBVRE E 1981 Froid et croissance de boutures de saules (Salix babylonica L.) cultivées in vitro. In Proc IUFRO Sect S2 01 5. Int Workshop “in Vitro” Cultivation for Tree Species, Fontainbleu, France, pp 115–118Google Scholar
  21. 21.
    GALZY R 1969 Recherches sur la croissance de Vitis rupestrls(Scheele) sain et court noue’ cultivé in vitro à different temperatures. Ann Phytopathol 1: 149–166Google Scholar
  22. 22.
    HELLERGREN J 1983 Cold acclimation of suspension cultures of Pinus sylvestris in response to light and temperature treatments. Plant Physiol 72: 992–995PubMedCrossRefGoogle Scholar
  23. 23.
    HIRAOKA N, T KODAMA 1984 Effects of non-frozen cold storage on the growth, organogenesis and secondary metabolism of callus cultures. Plant Cell Tiss Org Cult 3: 349–357CrossRefGoogle Scholar
  24. 24.
    HOWARD BH, HV OEHL 1981 Improved establishment of in vitro-propagated plum micropropagules following treatment with GA3 or prior chilling. J Hortic Sci 56: 1–7Google Scholar
  25. 25.
    HUNTER CS 1986 In vitro propagation and germplasm storage of Cinchona. In LA Withers, PG Alderton, eds. Plant Tissue Culture and its Agricultural Applications. Butterworths, England, pp 291–300Google Scholar
  26. 26.
    ILKER R, AJ WARING, JM LYONS, RW BREIDENBACH 1976 The cytological responses of tomato-seedling cotyledons to chilling and the influence of membrance modifications upon these responses. Protoplasma 90: 229–252CrossRefGoogle Scholar
  27. 27.
    KACPERSKA-PALACZ A 1978 Mechanism of cold acclimation in herbaceous plants. In PH Li, A SAKAI, eds. Plant Cold Hardiness and Freezing Stress. Academic Press, New York, pp 139–152Google Scholar
  28. 28.
    KARTHA KK 1981 Meristem culture and cryopreservation. In TA Thorpe, ed. Methods and Applications in Agriculture. Academic Press, New York, pp 181–211Google Scholar
  29. 29.
    LI PH, CJ WEISER 1971 Increasing cold resistance of stem sections of Cornus stolonifera by artificial dehydration. Cryobiology 8: 108PubMedCrossRefGoogle Scholar
  30. 30.
    LUNDERGAN C, J JANICK 1979 Low temperature storage of in vitro apple shoots. Hortic Sci 14: 514Google Scholar
  31. 31.
    LYONS JM 1973 Chilling injury in plants. Ann Rev Plant Physiol 24: 445–466CrossRefGoogle Scholar
  32. 32.
    MARINO G. P ROSATI. F SAGRATI 1985 Storage of in vitro cultures of Prunus rootstocks. Plant Cell Tiss Org Cult 5: 73–78CrossRefGoogle Scholar
  33. 33.
    MENZIES MI, DG HOLDEN, LM GREEN. DA ROOK 1981 Seasonal changes in frost tolerance of Pinus radiata seedlings raised in different nurseries. NZ J For Sci 11: 100–111Google Scholar
  34. 34.
    MULLIN RH, DE SCHLEGEL 1976 Cold storage maintenance of strawberry meristem plantlets. Hortscience 11: 100–101Google Scholar
  35. 35.
    PLATT-ALOIA KA, WW THOMSON 1976 An ultrastructural study of two forms of chilling-induced injury to the rind of grapefruit (Citrus paradisi. Mac. Fed.). Cryobiol 13: 95–106CrossRefGoogle Scholar
  36. 36.
    POISSONIER M. A FRANCLET, MJ DUMANT, JY GAUTRY 1980 Enracinement de tigelles in vitro de Sequoia sempervirens. Ann AFOCEL 1980: 231–253Google Scholar
  37. 37.
    RAISON JK. LC WRIGHT 1983 Thermal phase transitions in the polar lipids of plant membranes. Their induction by disaturated phospholipids and their possible relation to chilling injury. Biochem Biophys Acta 731: 69–78CrossRefGoogle Scholar
  38. 38.
    ROBARDS, AW, DT CLARKSON 1984 Effects Of chilling temperatures on root cell membrances as viewed by freeze-fracture electron microscopy. Protoplasma 122: 75–85CrossRefGoogle Scholar
  39. 39.
    RUGINI E, DC VERMA 1982 Micropropagation of difficult to propagate almond (Prunus amyqdalus) cultivar. Plant Sci Lett 28: 273–281Google Scholar
  40. 40.
    SAKAI A, Y SUGAWARA 1973 Survival of poplar callus at super-low temperatures after cold acclimation. Plant Cell Physiol 14: 1201–1204Google Scholar
  41. 41.
    SMITH DRS. KJ HORGAN, J AITKEN-CHRISTIE 1982 Micro-propagation of Pinus radiata for: afforestation. In A Fujiwara, ed. Plant Tissue Culture 1982. Japanese Assn for Plant Tissue Culture, Tokyo, pp 723–724Google Scholar
  42. 42.
    SUGAWARA Y, A SAKA1 1978 Cold acclimation of callus cultures of Jerusalem artichoke. In PH Li, A Sakai, eds. Plant Cold Hardiness and Freezing Stress. Academic Press, New York, pp 197–210Google Scholar
  43. 43.
    SUTTLE GRL 1983 Micropropagation of deciduous trees. In TH Hartmann, AT Leiser, W Roberts, eds, Int Plant Propagators Soc Combined Proc Vol 33. IPPS, Boulder, pp 46–49Google Scholar
  44. 44.
    SWEET GB, LG WELLS 1974 Comparison of the growth of vegetative propagules and seedlings of Pinus radiata. NZ J For Sci 4: 399–409Google Scholar
  45. 45.
    TOIVIO-KINNUCAN MA, HH CHEN, PH LI. C STUSHNOFF 1981 Plasma membrane alternations in callus tissues of tuber-bearing Solanum species during cold acclimation. Plant Physiol 67: 478–483PubMedCrossRefGoogle Scholar
  46. 46.
    VIEITEZ AM. A BALLESTER, ML VIEITEZ, E VIEITEZ 1983 In vitro plantlet regeneration of mature chestnut. J Hortic Sci 58: 457–463Google Scholar
  47. 47.
    VILLALOBOS VM 1983 The early events associated with organogenesis in cultured radiata pine cotyledons. PhD Thesis, Dep Biology, Univ Calgary, Canada, pp 69–71Google Scholar
  48. 48.
    WANAS WH, JA CALLOW, L WITHERS 1986 Growth limitation for the conservation of pear genotypes. In LA Withers. PG Alderton, eds. Plant Tissue Culture and its Agricultural Applications. Butterworths, England, pp 285–290Google Scholar
  49. 49.
    WILKINS CP, JH DODDS 1983 Tissue culture conservation of woody species. In JH Dodds, ed. Tissue Culture of Trees. AVI Publishing Co Inc. Connecticut, pp 113–136Google Scholar
  50. 50.
    WILKINS CP, JH DODDS 1985 Storage in vitro of temperate fruit trees. Int Board Plant Genet Res Newslett (in press)Google Scholar
  51. 51.
    WITHERS LA 1978 A fine-structural study of the freeze-preservation of plant tissue cultures. II. The thawed state. Protoplasma 94: 235–247CrossRefGoogle Scholar
  52. 52.
    WITHERS LA 1982 Storage of plant tissue cultures. In LA Withers, JT Williams, eds. Crop Genetic Resources — The Conservation of Difficult Material. IUBS/IBPGR, Paris, pp 49–82Google Scholar
  53. 53.
    WITHERS LA 1984 Freeze preservation of cells. In IK Vasil, ed. Cell Culture and Somatic Cell Genetics of Plants Vol 1. Academic Press, Florida, pp 608–620Google Scholar
  54. 54.
    WITHERS LA, MR DAVEY 1978 A fine-structural study of the freeze preservation of plant tissue cultures. I. The frozen state. Protoplasma 94: 207–209CrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishers, Dordrecht 1987

Authors and Affiliations

  1. 1.Forest Research InstituteRotoruaNew Zealand

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