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Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer

  • U. Schreiber

Abstract

A newly developed modulation fluorometer is described which operates with 1 µsec light pulses from a light-emitting diode (LED) at 100 KHz. Special amplification circuits assure a highly selective recording of pulse fluorescence signals against a vast background of non-modulated light. The system tolerates ratios of up to 1:107 between measuring light and actinic light. Thus it is possible to measure the “dark fluorescence yield” and record the kinetics of light-induced changes. A high time resolution allows the recording of the rapid relaxation kinetic following a saturating single turnover flash. Examples of system performance are given. It is shown that following a flash the reoxidation kinetics of photosystem II acceptors are slowed down not only by the inhibitor DCMU, but by a number of other treatments as well. From a light intensity dependency of the induction kinetics the existence of two saturated intermediate levels (I1 and I2) is apparent, which indicates the removal of three distinct types of fluorescence quenching in the overall fluorescence rise from F0 to Fmax.

Key words

chlorophyll fluorescence fluorometer fluorescence quenching Kautsky effect photosynthesis photosystem II 

Abbreviations

QA and QB

consecutive electron acceptors of photosystem II

PS II

photosystem II

P 680

reaction center chlorophyll of photosystem II

F0

minimum fluorescence yield following dark adaptation

Fmax

maximum fluorescence yield

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethyl-urea

DCCD

N,N′-dicyclohexylcarbodiimide

PQ

plastoquinone

DAD

diaminodurene

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References

  1. 1.
    Amesz J and Duysens LNM (1977) In primary processes of photosynthesis (Barber J,ed.) pp. 149–185 Amsterdam: ElsevierGoogle Scholar
  2. 2.
    Azzi A, Casey RP and Nalecz MJ (1984) Biochim Biophys Acta 768: 149–185Google Scholar
  3. 3.
    Bouges-Bocquet B (1973) Biochim Biophys Acta 314: 250–256PubMedCrossRefGoogle Scholar
  4. 4.
    Bowes JM and Crofts AR (1980) Biochim Biophys Acta 590: 373–384PubMedCrossRefGoogle Scholar
  5. 5.
    Bradbury M and Baker NR (1981) Biochim Biophys Acta 63: 542–551Google Scholar
  6. 6.
    Bradbury M and Baker NR (1984) Biochim Biophys Acta 765: 275–281CrossRefGoogle Scholar
  7. 7.
    Butler WL (1972) Proc Nat Acad Sci US 69: 3420–3422CrossRefGoogle Scholar
  8. 8.
    Crofts AR and Wraight CA (1983) Biochim Biophys Acta 726: 149–185Google Scholar
  9. 9.
    Delosme R (1967) Biochim Biophys Acta 143: 108–128PubMedCrossRefGoogle Scholar
  10. 10.
    Den Haan GA, Gorter de Vries H and Duysens LNM (1976) Biochim Biophys Acta 430:265–281CrossRefGoogle Scholar
  11. 11.
    Dietz K-J, Schreiber U and Heber U (1985) Planta, in pressGoogle Scholar
  12. 12.
    Duysens LNM and Sweers HE (1963) In studies on microalgae and photosynthetic bacteria, pp. 353–372. Tokyo: University of Tokyo PressGoogle Scholar
  13. 13.
    Duysens LNM, den Haan GA and van Best JA (1975) In Proc 3rd Int Congr Photosynth (Avron M, ed.) Vol 1, pp. 1–12. Amsterdam: ElsevierGoogle Scholar
  14. 14.
    Heber U and Santarius KA (1970) Z Naturforsch 25b: 718–728Google Scholar
  15. 15.
    Heber U (1973) Biochim Biophys Acta 305: 140–152PubMedCrossRefGoogle Scholar
  16. 16.
    Jensen RG and Bassham JA (1966) Proc Nat Acad Sci US 56: 1095–1101CrossRefGoogle Scholar
  17. 17.
    Joliot A (1974) In Proc 3rd Congr Photosynth Res, Rehovot (Avron M, ed.) Vol 1, pp. 315–322, Elsevier: AmsterdamGoogle Scholar
  18. 18.
    Kautsky H, Appel W and Amann (1960) Biochem Z 332: 277–292PubMedGoogle Scholar
  19. 19.
    Klimov W, Klevanik AV, Shuvalov VA and Krasnovskii AA (1977) FEBS Lett 82: 183–186PubMedCrossRefGoogle Scholar
  20. 20.
    Krause GH, Briantais JM and Vernotte C (1982) Biochim Biophys Acta 679: 116–124CrossRefGoogle Scholar
  21. 21.
    Krause GH and Weis E (1984) Photosynth Res 5: 139–157CrossRefGoogle Scholar
  22. 22.
    Kyle DJ, Ohad I and Arntzen CJ (1984) Proc Nat Acad Sci US 81: 4070–4074CrossRefGoogle Scholar
  23. 23.
    Laasch H, Schreiber U and Urbach W (1983) FEBS Lett 159: 275–279CrossRefGoogle Scholar
  24. 24.
    Lavergne J (1982) Biochim Biophys Acta 682: 345–353CrossRefGoogle Scholar
  25. 25.
    Lavorel J and Etienne AL (1977) In primary processes of photosynthesis (Barber J, ed.) pp. 203–268. Amsterdam: ElsevierGoogle Scholar
  26. 26.
    Mauzerall D (1972) Proc Nat Acad Sci US 69: 1358–1362CrossRefGoogle Scholar
  27. 27.
    Papageorgiou G (1975) In bioenergetics of photosynthesis (ed. Govindjee) pp. 319–371. New York: Academic PressGoogle Scholar
  28. 28.
    Quick WP and Horton P (1984) Proc R Soc Lond B 220: 371–382CrossRefGoogle Scholar
  29. 28.
    Quick WP and Horton P (1984) Proc R Soc Lond B 220: 371–382CrossRefGoogle Scholar
  30. 30.
    Rienits KG, Hardt H and Avron M (1974) Eur J Biochem 43:291–298PubMedCrossRefGoogle Scholar
  31. 31.
    Sane PV, Johanningmeier U and Trebst A (1979) FEBS Lett 108: 136–140PubMedCrossRefGoogle Scholar
  32. 32.
    Schreiber U and Avron M (1979) Biochim Biophys Acta 546: 436–447PubMedCrossRefGoogle Scholar
  33. 33.
    Schreiber U (1983) Photosynth Res 4: 361–373Google Scholar
  34. 34.
    Schreiber U (1984) Biochim Biophys Acta 767: 70–79CrossRefGoogle Scholar
  35. 34.
    Schreiber U, Bilger W and Schliwa U (1985) Photosynth Res, in pressGoogle Scholar
  36. 36.
    Solioz M (1984) Trends Biochem Sci 9: 309–312CrossRefGoogle Scholar
  37. 37.
    Van Best (1977) Doctoral Thesis, State University of Leiden, the NetherlandsGoogle Scholar
  38. 38.
    Van Best JA and Duysens LNM (1977) Biochim Biophys Acta 459: 187–206PubMedCrossRefGoogle Scholar
  39. 39.
    Velthuys B and Amesz J (1974) Biochim Biophys Acta 333: 85–94PubMedCrossRefGoogle Scholar
  40. 40.
    Velthuys BR (1980) Ann Rev Plant Physiol 31: 545–567CrossRefGoogle Scholar
  41. 41.
    Velthuys BR (1981) FEBS Lett 126: 277–281CrossRefGoogle Scholar
  42. 42.
    Vermaas WFJ and Govindjee (1981) Photochem Photobiol 34: 775–793Google Scholar
  43. 43.
    Vernotte C, Etienne AL and Briantais J-M (1979) Biochim Biophys Acta 545: 519–527PubMedCrossRefGoogle Scholar
  44. 44.
    Yamashita T and Butler WL (1968) Plant Physiol 43: 2037–2040PubMedCrossRefGoogle Scholar
  45. 45.
    Zankel Kl (1973) Biochim Biophys Acta 325: 138–148PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff/Dr. W. Junk Publishers, Dordrecht 1986

Authors and Affiliations

  • U. Schreiber
    • 1
  1. 1.Lehrstuhl Botanik IUniversität WürzburgWürzburgGermany

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