Summary
The role of fimbriae in enterobacterial adhesion to roots of grasses and cereals is discussed. All nitrogen-fixing enteric bacteria isolated in Finland had fimbriae. All Enterobacter isolates had mannose-binding type-1 fimbriae, whereas most of the Klebsiella isolates had both type-1 and type-3 fimbriae. The strains were isolated from a total of ten different grass species, and no specific association was found between grass species and bacterial fimbriation, biogroup or serogroup. Purified, radiolabeled fimbriae bound to roots of Poa pratensis in vitro, and bacterial adhesion was inhibited by Fab fragments specific for fimbriae. Klebsiella strains carrying type-3 fimbriae adhered to roots of various grasses and cereals more efficiently than type-1- or nonfimbriated strains, and it was concluded that type-3 fimbriae are the major adhesions of Klebsiella. Immunofluorescence studies revealed that the bacteria preferentially adhered to root hairs, and to a lesser extent, to the zone of elongation and the root cap mucilage. No strict host specificity in enterobacterial adhesion was observed.
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References
Barak R, Nur I and Okon Y 1983 Detection of chemotaxis in Azospirillum brasilense. J. Appl. Bacteriol. 53, 399–403.
Dazzo F B 1981 Bacterial attachment as related to cellular recognition in the Rhizobium-legume symbiosis. J. Supramol. Struct. Cell. Biochem. 16, 29–41.
Duguid J P 1959 Fimbriae and adhesive properties in Klebsiella strains. J. Gen. Microbiol. 21, 271–286.
Duguid J P and Old D C 1980 Adhesive properties of Enterobacteriaceae, pp 185–217. In Bacterial Adherence, Receptors and Recognition. Ed. E H Beachey. Series B, Vol 6, Chapman and Hall, London.
Ewing W H and Fife M A 1972 Enterobacter agglomerans (Beijerinck) comb. nov. (the Herbicola-Lathyri bacteria). Int. J. Syst. Bacteriol. 22, 4–11.
Gaastra W and de Graaf F K 1982 Host-specific fimbrial adhesins of noninvasive entero-toxigenic Escherichia coli strains. Microbiol. Rev. 46, 129–161.
Haahtela K and Korhonen T K 1984 In vitro adhesion of N2-fixing enteric bacteria to roots of grasses and cereals. Appl Environm. Microbiol. 49, 1186–1190.
Haahtela K, Tarkka E and Korhonen T K 1984 Type-1-fimbriae-mediated adhesion of enteric bacteria to grass roots. Appl. Environm. Microbiol. 49, 1182–1185.
Hrabak E M, Urbano M A and Dazzo F B 1981 Growth-phase-dependent immunodeterminants of Rhizobium trifolii lipopolysaccharide which binds Trifoliin A, a white clover lectin. J. Bacteriol. 148, 697–711.
Kijne J W, van der Schaal CAM, Diaz C L and van Iren F 1983 Mannose-specific lectins and the recognition of pea roots by Rhizobium leguminosarum. pp 521–530, In Lectins, Vol 3. Eds T C Bog-Hansen and G A Spleger. W de Gruyter and Co, Berlin-New York.
Korhonen T K 1979 Yeast cell agglutination by purified enterobacterial pili. FEMS Microbiol. Lett. 6, 421–425.
Korhonen T K, Tarkka E, Ranta H and Haahtela K 1983 Type 3 fimbriae of Klebsiella sp: Molecular characterization and role in bacterial adhesion to plant roots. J. Bacteriol. 155, 860–865.
Korhonen T K, Väisänen-Rhen V, Rhen M, Pere A, Parkkinen J and Finne J 1984 Escherichia coli fimbriae recognizing sialyl galactosides. J. Bacteriol. 159, 762–766.
Korhonen T K, Väisänen V, Saxen H, Hultberg H and Svenson S B 1982 P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains. Infect. Immun. 37, 286–291.
Lippincot B B, Whatley M H and Lippincot J A 1977 Tumor induction by Agrobacterium involves attachment of the bacterium to a site on the host plant cell wall. Plant Physiol. 59, 388–390.
Nowicki B, Rhen M, Väisänen-Rhen V, Pere A and Korhonen T K 1984 Immunofluorescence study of fimbrial phase variation in Escherichia coli KS71. J. Bacteriol. 160, 691–291.
Ofek I, Mirelman D and Sharon N 1977 Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature (London) 265, 623–625.
Old D C 1972 Inhibition of the interaction between fimbrial hemagglutinins and erythrocytes by D-mannose and other carbohydrates. J. Gen. Microbiol. 71, 149–157.
Old D C and Adegbola R A 1984 A comparative immunoelectronmicroscopical study of fimbriae of Enterobacter and Klebsiella. System. Appl. Microbiol. 5, 157–168.
Patriquin DG, Döbereiner J and Jain D K 1983 Sites and processes of association between diazotrophs and grasses. Can. J. Microbiol. 29, 900–915.
Rhen M, Klemm P, Wahlström E, Svenson S B, Källenius G and Korhonen T K 1983 P-fimbriae of Escherichia coli: immuno- and protein-chemical characterization of fimbriae from two pyelonephritogenic strains. FEMS Microbiol. Lett. 18, 233–238.
Rhen M, Knowles J, Penttilä M E, Sarvas M and Korhonen T K 1983 P fimbriae of Escherichia coli: molecular cloning of DNA fragments containing the structural genes. FEMS Microbiol. Lett. 19, 119–123.
Rhen M, Mäkelä P H and Korhonen T K 1983 P fimbriae of Escherichia coli are subject to phase variation. FEMS Microbiol. Lett. 19, 267–271.
Umali-Garcia M, Hubbel D H, Gaskins M H and Dazzo F B 1980 Association of Azospirillum with grass roots. Appl. Environ. Microbiol. 39, 219–226.
Vesper S J and Bauer W D 1984 Role of pili in Rhizobium attachment. Proceedings of the Second International Symposium on the Molecular Genetics of Bacteria-Plant Interaction, Abstract No 99, Ithaca, USA.
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© 1986 Martinus Nijhoff Publishers, Dordrecht
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Korhonen, T.K., Nurmiaho-Lassila, EL., Laakso, T., Haahtela, K. (1986). Adhesion of fimbriated nitrogen-fixing enteric bacteria to roots of grasses and cereals. In: Skinner, F.A., Uomala, P. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 21. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4378-0_5
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DOI: https://doi.org/10.1007/978-94-009-4378-0_5
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