Summary
Labelling experiments with 15N glutamate and 15N alanine were conducted using slices from oat leaves to investigate photorespiratory nitrogen metabolism. It is concluded from the labelling kinetics of glutamine that the refixation of photorespiratory ammonia primarily occurs by glutamine synthetase in the chloroplast. The labelling kinetics of glutamine with 15N glutamate indicate that the chloroplastic and cytoplasmic glutamate pools do not exchange easily in oat leaf cells. Alanine was shown to be an important amino donor for photorespiratory glycine formation. This result is discussed with regard to a possible role of alanine in photorespiration. A modification to the scheme of photorespiratory nitrogen metabolism is proposed.
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Abbreviations
- GS:
-
Glutamine synthetase E.C. 6.3.1.2
- GOGAT:
-
Glutamate synthase E.C. 1.4.7.1
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© 1986 Martinus Nijhoff Publishers, Dordrecht/Boston/Lancaster
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Betsche, T., Eising, R. (1986). Refixation of photorespiratory ammonia and the role of alanine in photorespiration: Studies with 15N. In: Lambers, H., Neeteson, J.J., Stulen, I. (eds) Fundamental, Ecological and Agricultural Aspects of Nitrogen Metabolism in Higher Plants. Developments in Plant and Soil Sciences, vol 19. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4356-8_46
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DOI: https://doi.org/10.1007/978-94-009-4356-8_46
Publisher Name: Springer, Dordrecht
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Online ISBN: 978-94-009-4356-8
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