Nitrogen metabolism in plastids of pea roots

Emes, M. J.; England, S.

doi:10.1007/978-94-009-4356-8_24

Nitrogen metabolism in plastids of pea roots

M. J. Emes⁴ &
S. England⁴

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Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 19))

Summary

A method is described for the purification of root plastids by centrifugation through Percoll. Organelle marker enzyme distribution indicates a recovery of 30% with only 5% enrichment by other fractions. GOGAT latency indicates > 90% intactness. Less than 10% of total glutamine synthetase activity is associated with the preparation, the significance of which is discussed.

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References

Allen J F 1977 FEBS Lett 84, 221–224.
Article PubMed CAS Google Scholar
Crane F L 1957 Plant Physiol. 32, 619–625.
Article PubMed CAS Google Scholar
Dry I, Wallace W and Nicholas D J D 1981 Planta 152, 234–238.
Article CAS Google Scholar
Ernes M J and Fowler M W 1979 Planta 144, 249–253.
Article Google Scholar
Ernes M J and Fowler M W 1983 Planta 158, 97–102.
Article Google Scholar
Heber U and Purczeld P 1977 Proc. Fourth. Int. Cong, on Photosynthesis. Eds. D O Hall, J Coombs, T W Goodwin, London: The Biochemical Society pp 107–118.
Google Scholar
Hirel B, McNally S F, Gadal P, Sumar N and Stewart G R 1984 Eur. J. Biochem. 138, 63–66.
Article PubMed CAS Google Scholar
McNally S F, Hirel B, Gadal P, Mann F and Stewart G R 1983 Plant Physiol. 72, 22–25.
Article PubMed CAS Google Scholar
Miflin B J 1974 Plant Physiol. 54, 550–555.
Article PubMed CAS Google Scholar
Miflin B J and Lea P J 1980 In The Biochemistry of Plants, Ed. B J Miflin. Vol 5 pp 169–202
Google Scholar
Mills W R and Joy K W 1980 Planta 148, 75–83.
Article CAS Google Scholar
Robinson S P and Wiskich J T 1977 Plant Physiol. 59, 422–427.
Article PubMed CAS Google Scholar
Sarkissian G S and Fowler M W 1974 Planta 119, 335–349.
Article CAS Google Scholar
Stewart G R and Rhodes D 1977 New Phytol. 79, 257–268.
Article CAS Google Scholar
Suzuki A, Gadal P and Oaks A 1981 Planta 151, 457–461.
Article CAS Google Scholar

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Authors and Affiliations

Department of Botany, Manchester University, Manchester, UK
M. J. Emes & S. England

Authors

M. J. Emes
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S. England
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Editors and Affiliations

Department of Plant Ecology, University of Utrecht, Utrecht, The Netherlands
H. Lambers
Institute for Soil Fertility, Haren, The Netherlands
J. J. Neeteson
Department of Plant Physiology, University of Groningen, Haren, The Netherlands
I. Stulen

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Emes, M.J., England, S. (1986). Nitrogen metabolism in plastids of pea roots. In: Lambers, H., Neeteson, J.J., Stulen, I. (eds) Fundamental, Ecological and Agricultural Aspects of Nitrogen Metabolism in Higher Plants. Developments in Plant and Soil Sciences, vol 19. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4356-8_24

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DOI: https://doi.org/10.1007/978-94-009-4356-8_24
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8437-6
Online ISBN: 978-94-009-4356-8
eBook Packages: Springer Book Archive

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