Batch laboratory digesters were utilized to study the biogas production from digestion of cow manure contaminated with procaine penicillin (PP), ampicillin (AMP), tetracycline hydrochloride (TC.H), oxytetracycline (OTC), oxytetracycline hydrochloride (OTC.H), and chloramphenicol (CAM). Doses of the antibiotics added to the cow manure were based on the average recommended therapeutic dose. The digesters were fed with cow manure slurry of 5 percent TS and incubated at 35°C for 30 days.
The results showed that all the tested antibiotics, over the range of concentrations used, have an inhibitory effect when expressed as a reduction in the biogas volume produced. However, the degree of inhibition varied according to type of antibiotic, its concentration and the period of digestion.
Results of the effect of a constant dose (12.5 mg antibiotic per liter feed slurry) indicated that, in terms of the reduction in biogas volume produced within 30 days, OTC, AMP, and OTC.H were the most effective antibiotics; PP and CAM were the least effective, while TC.H had no effect. However, the tested antibiotics varied in their effect on the methane content of the biogas. Therefore, in terms of reduction in the volume of methane produced within 30 days, the tested antibiotics could be ranked in a decreasing order as follows: AMP, OTC, OTC.H, PP, CAM, and TC.H. The corresponding reduction percentages were 33, 32, 25, 14, 11, and 10 respectively.
- Anaerobic Digestion
- Methane Production
- Biogas Production
- Volatile Solid
- Methane Content
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