Biogas Production from Antibiotic-Contaminated Cow Manure

  • H. Gamal-El-Din


Batch laboratory digesters were utilized to study the biogas production from digestion of cow manure contaminated with procaine penicillin (PP), ampicillin (AMP), tetracycline hydrochloride (TC.H), oxytetracycline (OTC), oxytetracycline hydrochloride (OTC.H), and chloramphenicol (CAM). Doses of the antibiotics added to the cow manure were based on the average recommended therapeutic dose. The digesters were fed with cow manure slurry of 5 percent TS and incubated at 35°C for 30 days.

The results showed that all the tested antibiotics, over the range of concentrations used, have an inhibitory effect when expressed as a reduction in the biogas volume produced. However, the degree of inhibition varied according to type of antibiotic, its concentration and the period of digestion.

Results of the effect of a constant dose (12.5 mg antibiotic per liter feed slurry) indicated that, in terms of the reduction in biogas volume produced within 30 days, OTC, AMP, and OTC.H were the most effective antibiotics; PP and CAM were the least effective, while TC.H had no effect. However, the tested antibiotics varied in their effect on the methane content of the biogas. Therefore, in terms of reduction in the volume of methane produced within 30 days, the tested antibiotics could be ranked in a decreasing order as follows: AMP, OTC, OTC.H, PP, CAM, and TC.H. The corresponding reduction percentages were 33, 32, 25, 14, 11, and 10 respectively.


Anaerobic Digestion Methane Production Biogas Production Volatile Solid Methane Content 
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  1. American Public Health Association. 1980. Standard Methods for the Examination of Water and Wastewater ( 15th ed. ). APHA. Washington, D.C.Google Scholar
  2. Brander, G.C. and D.M. Pugh. 1977. Veterinary Applied Pharmacology and Therapeutics. ( 3rd ed. ). ELBS and Bailliere Tindall. London.Google Scholar
  3. Bryant, M.P. 1979. Microbial Methane production: Theoretical aspects. J. Animal Sci. 48: 193–201.Google Scholar
  4. Clark, C.E. 1965. Hog waste disposal by lagooning. Amer. Soc. Civil Eng. 91: 27–30.Google Scholar
  5. Gamal-El-Din, H. 1984. Biogas from organic waste diluted with sea-water. Proc. International Conf. “State of the Art on Biogas Technology, Transfer and Diffusion”. Cairo, Egypt, November 17–24, 1984.Google Scholar
  6. Hobson, P.N. and B.G. Show. 1976. Inhibition of methane production by ethanobacterium formicicum. Water Res. 10: 849–852.CrossRefGoogle Scholar
  7. Hobson, P.N., S. Bousfield, and R. Summers. 1981. Methane Production from Agricultural and Domestic Wastes. Applied Science Publishers, Ltd. London.Google Scholar
  8. Langlois, B.E., G.L. Cromwell and V.W. Hays. 1978. Influence of chlortetracycline in swine feed on reproductive performance and on incidence and persistence of antibiotic resistant enteric bacteria. J. Animal Sci. 46: 1369–1382.Google Scholar
  9. Morrison, S.M., D.W. Grant, M.P. Nevins, and G.K. Elmund. 1969. Role of Excreted Antibiotic in Modifying Microbial Decomposition in Feedlot Waste. Proc. Cornell Univ. Agr. Waste Manag. Conf. Syracuse, N.Y.Google Scholar
  10. Patten, D.K. and D.C. Wolf. 1979. Effect of Antibiotics in Beef Feces on Nitrogen and Carbon Mineralization in Soil. Agronomy Abstracts, 1979. Ann. Meeting. Colorado University.Google Scholar
  11. Stafford, D.A., D.L. Howkes, and R. Horton. 1980. Methane Production from Waste Organic Matter. CRC Press, Inc. Boca Raton, Florida.Google Scholar
  12. Wise, D.L., R.L. Wentworth, and R.G. Kispert. 1976. Fuel Gas Production From Selected Bioraass Via Anaerobic Fermentation. Presented at the U.S.-Japan Joint Cooperative Seminar Entitled “Biological Solar Energy Conversion.” Miami, Florida, November 15–18, 1976.Google Scholar

Copyright information

© Elsevier Applied Science Publishers Ltd 1986

Authors and Affiliations

  • H. Gamal-El-Din
    • 1
  1. 1.Faculty of AgricultureCairo UniversityFayumEgypt

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