Abstract
Direct immunofluorescent staining of tissue impression smears is a rapid, specific diagnostic test for avian influenza, which is as sensitive as virus isolation in chick embryos. A FITC-conjugated antiserum prepared against one subtype of influenza virus stains antigenically unrelated subtypes. Diagnosis by inoculation of chick cell cultures is insensitive compared with immunofluorescence or embryo inoculation.
The protection induced in chickens by live neuraminidase (N)-specific vaccines against challenge with highly pathogenic avian influenza viruses was investigated. Vaccination with viruses belonging to N1 and N8 subtypes conferred protection against challenge with viruses of the same N subtype and irrelevant haemagglutinin (H) subtypes. A particular advantage of N-specific vaccines is that they do not interfere with serological diagnosis by haemagglutination inhibition tests. The anomalous cross-protection between viruses of H1 and H5 subtypes could be exploited to control outbreaks of disease caused by highly pathogenic H5 viruses by using vaccines based on H1 and the appropriate N antigen.
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© 1986 ECSC, EEC, EAEC, Brussels-Luxembourg
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McNulty, M.S., McFerran, J.B. (1986). Avian Influenza: Diagnosis and Vaccination. In: McFerran, J.B., McNulty, M.S. (eds) Acute Virus Infections of Poultry. Current Topics in Veterinary Medicine and Animal Science, vol 37. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4287-5_4
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DOI: https://doi.org/10.1007/978-94-009-4287-5_4
Publisher Name: Springer, Dordrecht
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