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New Possibilities for Cytogenetic Analysis of Leukemic Cells

  • A. Hagemeijer
  • H. J. Adriaansen
  • C. R. Bartram
Part of the Developments in Oncology book series (DION, volume 45)

Abstract

The first cytogenetic aberration associated with leukemia has been reported more than 25 years ago, i.e. the Philadelphia (Ph) chromosome in chronic myeloid leukemia (CML) (1). At the present time, it appears that most, if not all cases of acute leukemia are characterized by cytogenetic changes which follow a non-random pattern of occurrence (2,3). Almost every month, a new translocation or chromosomal rearrangement is described as specifically associated with a subtype of leukemia with characteristic clinical, pathological and immunological features (Table I) (3–7). In contrast with the immunological markers which are differentiation markers that appear also on some normal cells, cytogenetic markers are unique and specific features of leukemic cells. It may be expected within a few years that a chromosomal classification of hematological malignancies will develop next to the FAB classification presently in use (8,9).

Keywords

Chronic Myeloid Leukemia Acute Leukemia Minimal Residual Disease Acute Nonlymphocytic Leukemia Cellular Oncogene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Nowell PC, Hungerford DA. A minute chromosome in human chronic granulocytic leukemia. Science 132, 1497 (1960).Google Scholar
  2. 2.
    Sandberg AA. The chromosomes in human cancer and leukemia. Elsevier North Holland, New York (1980).Google Scholar
  3. 3.
    Yunis JJ. The chromosomal basis of human neoplasia. Science 221: 227–236 (1983).PubMedCrossRefGoogle Scholar
  4. 4.
    Yunis JJ, Brunning RD, Howe RB, Lobell M. High-resolution chromosomes as an independent prognostic indicator in adult acute nonlymphocytic leukemia. New Engl J Med 311: 812–818 (1984).PubMedCrossRefGoogle Scholar
  5. 5.
    Williams DL, Look AT, Melvin SL, Robertson PK, Dahl G, Flake T, Stass s. New chromosomal translocations with specific immunophenotypes of childhood acute lymphoblastic leukemia. Cell 36: 101–109 (1984).PubMedCrossRefGoogle Scholar
  6. 6.
    Fourth International workshop on chromosomes in leukemia A prospective study of acute non-lymphocytic leukemia. Cancer Genet.Cytogenet. 11: 249–360 (1984).Google Scholar
  7. 7.
    Mitelman F. Catalogue of chromosome aberrations in cancer. Cytogenet.Cell Genet. 36: 1–515 (1983).PubMedCrossRefGoogle Scholar
  8. 8.
    Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, Sultan C. Proposals for the classification of the acute leukaemias. Br.J.Haematol. 33: 451–458 (1976).PubMedCrossRefGoogle Scholar
  9. 9.
    Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Galnick HR, Sultan C. Proposals for the classification of the myelodysplastic syndromes. Brit.J.Haematol. 51: 189–199 (1982).Google Scholar
  10. 10.
    ISCN. An International System for Human Cytogenetic Nomenclature. Cytogenet.Cell Genet. 21: 309–404 (1978).CrossRefGoogle Scholar
  11. 11.
    Heisterkamp N, Groffen J, Stephenson JR, Spurr NK, Goodfellow PN, Solomon E, Carrit B, Bodmer WF. Chromosomal localization of human cellular homologues of two viral oncogenes. Nature 299: 747–749 (1982).PubMedCrossRefGoogle Scholar
  12. 12.
    De Taisne C, Gegonne A, Stehelin D, Bernheim A, Berger R. Chromosomal localization of the human proto-oncogene c-ets. Nature 310: 581–583 (1984).PubMedCrossRefGoogle Scholar
  13. 13.
    De Klein A, Hagemeijer A. Cytogenetic and molecular analysis of the Phl translocation in chronic myeloid leukemia. Cancer Surveys 3: 515–529 (1984).Google Scholar
  14. 14.
    Nowell PC, Erikson J, Finan J, Emanuel B, Croce CM. Chromosomal translocations, immunoglobulin genes and oncogenes in human B-cell tumours. Cancer Surveys 3: 531–541 (1984).Google Scholar
  15. 15.
    Harper ME, Saunders GE. Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83: 431–439 (1981).PubMedCrossRefGoogle Scholar
  16. 16.
    le Beau M, Diaz MO, Rowley JD, Mak TW. Chromosomal localization of the human T cell receptor B chain genes. Cell 41: 335 (1985).PubMedCrossRefGoogle Scholar
  17. 17.
    Sandberg AA. Chromosomes and causation of human cancer and leukemia: XL. The Ph′ and other translocations in CML. Cancer 46: 2221–2226 (1980).PubMedCrossRefGoogle Scholar
  18. 18.
    William CP, Pearson M, Vardiman JW, Rowley JD. Philadelphia chromosome-negative chronic myelogenous leukaemia: a morphological reassessment. Brit.J.Hematol. 60: 457–467 (1985).CrossRefGoogle Scholar
  19. 19.
    De Klein A, Geurts van Kessel A, Grosveld G, Bartram CR, Hagemeijer A, Bootsma D, Spurr NK, Heisterkamp N, Groffen J, Stephenson JR. A cellular oncogene is translocated to the Philadelphia chromosome in chronic myelocytic leukaemia. Nature 300: 765–767 (1982).PubMedCrossRefGoogle Scholar
  20. 20.
    Groffen J, Heisterkamp N, Stephenson JR, Geurts van Kessel A, de Klein A, Grosveld G, Bootsma D. c-sis is translocated from chromosome 22 to chromosome 9 in chronic myelocytic leukemia. J.Exp.Med. 158: 9–15 (1983).PubMedCrossRefGoogle Scholar
  21. 21.
    Bartram CR, de Klein A, Hagemeijer A, van Agthoven T, Geurts van Kessel A, Bootsma D, Grosveld G, Davies T, Stone M, Ferguson-Smith MA, Heisterkamp N, Groffen J, Stephenson JR. Translocation of the human c-abl oncogene occurs in variant Ph1-positive but not Ph1-negative chronic myelocytic leukaemia. Nature 306: 277–280 (1983).PubMedCrossRefGoogle Scholar
  22. 22.
    Hagemeijer A, Bartram CR, Smit EME, van Agthoven AJ, Bootsma D. Is the chromosomal region 9q34 always involved in variants of the Ph′ translocation? Cancer Genet Cytogenet 13: 1–16 (1984).PubMedCrossRefGoogle Scholar
  23. 23.
    Hagemeijer A, de Klein A, Gödde-Salz E, Turc-Carel C, Smit EME, van Agthoven AJ, Grosveld GC. Translocation of c-abl to ‘masked’ Ph in chronic myeloid leukemia. Cancer Genet. Cytogenet. in press (1985).Google Scholar
  24. 24.
    Bartram CR, de Klein A, Hagemeijer A, Grosveld G, Heisterkamp N, Groffen J. Localization of the human c-sis oncogene in Ph1-positive and Ph1-negative chronic myelocytic leukemia by in situ hybridization. Blood 63: 223–225 (1984).PubMedGoogle Scholar
  25. 25.
    Groffen J, Stephenson JR, Heisterkamp N, de Klein A, Bertram CR, Grosveld G. Clustering of Philadelphia chromosomal breakpoints within a limited region bcr, on chromosome 22. Cell 36: 93–99 (1984).PubMedCrossRefGoogle Scholar
  26. 26.
    Bartram CR, Kleihauer E, de Klein A, Grosveld G, Teyssier JR, Heisterkamp N, Groffen J. c-abl and bcr are rearranged in a Ph′-negative CML patient. EMBO J 4: 683–686 (1985).Google Scholar
  27. 27.
    Hagemeijer A, Smit EME, Bootsma D. Improved identification of chromosomes of leukemic cells in methotrexate treated cultures. Cytogenet. Cell Genet. 23: 208–212 (1979).PubMedCrossRefGoogle Scholar
  28. 28.
    Webber LM, Garson OM. Fluorodeoxyuridine synchronization of bone marrow cultures. Cancer Genet.Cytogenet. 8: 123–132 (1983).PubMedCrossRefGoogle Scholar
  29. 29.
    Testa JR. High resolution chromosomal analysis of acute leukemia: current assessment. Cancer Surveys 3: 359–369 (1984).Google Scholar
  30. 30.
    Berger R, Flandrin G. Determining the nature of cells studied cytogenetically. Cancer Surveys 3: 423–438 (1984).Google Scholar
  31. 31.
    Teerenhovi L, Knuutila S, Ekblom M, Rossi L, Bergstrom GH, Talman JK, Andersson L, Chapelle A de la. A method for simultaneous study of the karyotype, morphology and immunologic phenotype of mitotic cells in hematologic malignancies. Blood 64: 1116–1127 (1984).PubMedGoogle Scholar
  32. 32.
    Knuutila S, Keinänen M. Chromosome banding techniques for morphologically classified cells. Cytogenet. Cell Genet. 39: 70–72 (1985).PubMedCrossRefGoogle Scholar
  33. 33.
    Knapp W, Majdic O, Bettelheim P, Liszka K, Stockinger H. Antigenic heterogeneity in acute leukemia. Contr.Oncol. 19: 75–87 (1984) (Karger, Basel).Google Scholar

Copyright information

© Martinus Nijhoff Publishers, Dordrecht 1986

Authors and Affiliations

  • A. Hagemeijer
  • H. J. Adriaansen
  • C. R. Bartram

There are no affiliations available

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