Abstract
Isolated plant protoplasts are ‘naked’ cells that have had their cell wall removed either by mechanical action or by enzymic digestion. As a result of wall removal the only barrier that exists between the cell protoplasm and the external environment is the plasma membrane. The removal of the cell wall has drastic osmotic consequences for the isolated cell. Rather like animal cells, the isolated protoplasts must be maintained in an osmotically balanced (isotonic) nutrient medium or they will burst.
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© 1986 Teresa Bengochea and John H. Dodds
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Bengochea, T., Dodds, J.H. (1986). Introduction. In: Plant Protoplasts. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4095-6_1
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DOI: https://doi.org/10.1007/978-94-009-4095-6_1
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8317-1
Online ISBN: 978-94-009-4095-6
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