Tissue and cell culture of New Zealand Pterocladia and Porphyra species

  • Liu Xue-wu
  • Margaret E. Gordon
Conference paper
Part of the Developments in Hydrobiology book series (DIHY, volume 41)


Seaweeds are not currently cultivated in New Zealand, where bacteriological agar is produced from wild populations of Pterocladia lucida and P. capillacea. The possibility of harvesting and processing New Zealand Gracilaria is being considered, and the potential for mariculture of agar seaweeds is being examined (Christeller et al., 1983; Marsh, 1985). Our research on algal tissue culture has accordingly been directed towards developing protoplast and micropropagation techniques for New Zealand agarophytes. Our current emphasis is on Pterocladia, but we are also working on Porphyra columbina because Porphyra species have proved amenable to tissue and protoplast culture in other studies (e.g., Polne-Fuller amp; Gibor, 1984 and references therein).

Key words

seaweed Pterocladia Porphyra cell separation callus hormones 


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  1. Atasuka, I., 1978. Hair and cuticle as special structure of frond structure of Gelidiaceous algae. Rev. algol. 13: 349 – 358.Google Scholar
  2. Bradley, P. M. amp; D. P. Cheney, 1986. Morphogenetic variation in tissue cultures of the red seaweed Agardhiella subulata. Amer. J. Bot. 73: 649.Google Scholar
  3. Cheney, D. P., A. H. Luistro amp; P. M. Bradley, 1987. Carragee– nan analysis of tissue cultures and whole plants of Agardhiella subulata. Proc. int. Seaweed Symp. 12: 161–166.Google Scholar
  4. Christeller, J. T., R. H. Furneaux, M. E. Gordon, W. A. Laing, I. J. Miller, W. A. Nelson, B. D. Shaw amp; B. E. Terzaghi, 1983. The potential for the mariculture of seaweeds in New Zealand. Tech. Rep. 18, Plant Physiol. Div., DSIR, Palmerston North, New Zealand, 21 pp.Google Scholar
  5. Dromgoole, F. I. amp; W. E. Booth, 1985. The structure and development of hairs on the thallus of Gelidium caulacanthe– um. J. Ag. N. Z. J. mar. Freshw. Res. 19: 43–48.CrossRefGoogle Scholar
  6. Felicini, G. P. amp; O. Arrigoni, 1967. Richerche sulla rigenerazi– one in coltura di Pterocladia capillacea (Gmel.) Born, et Thur. Giorn. Bot. Ital. 101: 199–217.CrossRefGoogle Scholar
  7. Liu W. S., Tang Y. L., Liu X. W. amp; Fang T. C., 1984. Studies on the preparation and on the properties of sea snail enzymes. Proc. int. Seaweed Symp. 11: 319 – 320.Google Scholar
  8. McLachlan, J., 1973. Growth media – marine. In J. R. Stein (ed.), Handbook of Phycological Methods. Culture Methods and Growth Measurements. Cambridge Univ. Press, Cambridge: 25 – 51.Google Scholar
  9. Marsh, R. N., 1985. Opportunities for, and constraints on, commercial cultivation of Gracilaria: policy options. M.Sc. Thesis, Centre for Resource Management, Univ. Canterbury, Christchurch, New Zealand, 137 pp.Google Scholar
  10. Perrone, C. amp; G. P. Felicini, 1974. Dominance apicale et mor– phogénése chez Petroglossum nicaeense (Duby) Schotter ( Rhodophyceae ). Phycologia 13: 187–194.CrossRefGoogle Scholar
  11. Perrone-Pesola, C. amp; G. P. Felicini, 1981. Polarité dans la fronde de Schottera nicaeensis ( Phyllophoracées ). Phycologia 20: 142–146.CrossRefGoogle Scholar
  12. Polne-Fuller, M. amp; A. Gibor, 1984. Developmental studies in Porphyra. I. Blade differentiation in Porphyra perforata as expressed by morphology, enzymatic digestion and protoplast regeneration. J. Phycol. 20: 609 – 616.CrossRefGoogle Scholar
  13. Polne–Fuller, M., M. Biniaminov amp; A. Gibor, 1984. Vegetative propagation of Porphyra perforata. Proc. int. Seaweed Symp. 11: 308–313.Google Scholar
  14. Provasoli, L., 1968. Media and prospects for cultivation of marine algae. In A. Watanabe amp; A. Hattori (eds), Cultures and Collections of Algae (Proc. U.S. - Japan Conf., Hakone ), Jpn Soc. PI. Physiol.: 63 – 75.Google Scholar
  15. Tang, Y. L., 1982. Isolation and cultivation of the vegetative cells and protoplasts of Porphyra suborbiculata Kjellm. J. Shandong Coll. Oceanogr. 12(4): 37–50 (in Chinese with English abstract).Google Scholar
  16. Waaland, S. D. amp; J. R. Waaland, 1975. Analysis of cell elongation in red algae by fluorescent labelling. Planta (Berl.) 126: 127 – 138.CrossRefGoogle Scholar

Copyright information

© Dr W. Junk Publishers, Dordrecht 1987

Authors and Affiliations

  • Liu Xue-wu
    • 1
  • Margaret E. Gordon
    • 1
  1. 1.Botany DepartmentVictoria University of WellingtonWellingtonNew Zealand

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