Abstract
The aim of our work is to contribute to the elaboration of a reliable quantitative method for individual glucosinolates determination in rapeseeds.
To emphasize the accuracy of the method, we have studied several important points:
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a)
methanol - water (70%) extraction from seeds and seedcakes obtained after removal of the oil.
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b)
column chromatographic purification of crude extracts
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the influence of the height of D.E.A.E. A 25 columns (desulpho glucosinolates) and of Ecteolla Cellulose (intact glucosinolates) on the level of glucosinolates obtained is analysed
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to avoid the use of pyridin, the pyridin - acetate buffer frequently used to prepare D.E.A.E. columns is changed by an acetic acid - sodium acetate buffer (pH : 5)
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c)
HPLC analysis of desulphoglucosinolates and intact glucosinolates
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comparison of desulphoglucosinolates and intact glucosinolates separations
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choice of the detection wavelength
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influence of column temperature
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d)
Identification of desulphoglucosinolates from different cultivars of rapeseed (Garant, Ledos, Lirasol and Librador).
Keywords
- Column Chromatographic Purification
- Chemical Ionisation Spectrum
- Intact Glucosinolates
- Individual Glucosinolates
- Oxazolidine Thiones
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
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© 1987 ECSC, EEC, EAEC, Brussels-Luxembourg
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Wathelet, JP., Biston, R., Marlier, M., Séverin, M. (1987). Analysis of Individual Glucosinolates in Rapeseeds Comparison Between Different Methods. In: Wathelet, JP. (eds) Glucosinolates in Rapeseeds: Analytical Aspects. World Crops: Production, Utilization, Description, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3615-7_9
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DOI: https://doi.org/10.1007/978-94-009-3615-7_9
Publisher Name: Springer, Dordrecht
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