Abstract
A series of bacteriocin-insensitive and phage-resistant mutants of Erwinia chrysanthemi 3937J, which is pathogenic on Saintpaulia ionantha, were analyzed with regard to their detectable changes in outer membrane (OM) structure. Two types of OM alterations were detected: alterations (i) in low iron inducible proteins and (ii) in the lipopolysaccharide. A pleiotropic effect was observed in the phenotypes of the mutants. For the mutants which were tested on saintpaulia plants, a correlation could be seen between the loss of pathogenicity and the alterations in low iron inducible proteins. The mutants did not exhibit altered extracellular pectinolytic and cellulolytic activities.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bertheau Y, Madjidi-Hervan E, Kotoujansky A, Nguyen-The C, Andro T, Coleno A. 1984. Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves. Anal. Biochem. 139:383–389.
Carlson RW. 1984. Heterogeneity of Rhizobium lipopolysaccharides. J. Bacteriol. 158:1012–1017.
Chatterjee AK, Starr MP. 1980. Genetics of Erwinia species. Annu. Rev. Microbiol. 34:645–676.
Collmer AP, Berman P, Mount MS. 1982. Pectate lyase regulation and bacterial soft-rot pathogenesis. In: Mount M and Lacy G (eds.), Phytopathogenic Prokaryotes, Vol. 1, Academic Press, Inc., New York, pp. 395–422.
Dickey RS. 1981. Erwinia chrysanthemi: reaction of eight plant species to strains from several hosts and to strains of other Erwinia species. Phytopathology 71:23–29.
Garrett CME, Crosse JE, Sletten A. 1974. Relations between phage sensitivity and virulence in Pseudomonas morsprunorum. J. Gen. Microbiol. 80:457–83.
Goldman RC, Leive L. 1980. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 011 and Salmonella typhimurium LT12. Eur. J. Biochem. 107:145–153.
Hitchcock PJ, Brown T. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269–277.
Kamp D, Sandulache R. 1983. Recognition of cell surface receptors is controlled by invertible DNA of phage Mu. FEMS Microbiol. Lett. 16:131–135.
Leong SA, Neilands JB. 1981. Relationship of siderophore mediated iron assimilation to virulence in crown gall disease. J. Bacteriol. 147:482–491.
Leong SA, Neilands JB. 1982. Siderophore production by phytopathogenic microbial species. Arch. Biochem. Biophy. 218:351–359.
Neiland JB. 1982. Microbial envelope proteins related to iron. Annu. Rev. Microbiol. 36:285–309.
Pavia T, Makela PH. 1980. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl polyacrylamide gel electrophoresis. Eur. J. Biochem. 107:137–143.
O’Brien IG, Gibson F. 1970. The structure of enterochelin and related 2,3-dihydroxy-N-benzoylserine conjugated from Escherichia coli. Biochim. Biophys. Acta 215:393–402.
Pugsley AP, Reeves P. 1976. Characterization of group B colicin resistant mutants of Escherichia coli K-12: colicin resistance and the role of enterochelin. J. Bacteriol. 127:218–228.
Sandulache R, Prehms P, Kamp D. 1985. Cell wall receptor for bacteriophage Mu G(-) in Erwinia chrysanthemi and E. coli C. J. Bacteriol. In press.
Sequeira L, Graham TL. 1977. Interaction of bacteria and host cell walls: its relation to mechanisms of induced resistance. Physiol. Plant. Pathol. 11:43–54.
Weinberg E. 1978. Iron and infection. Microbiol. Rev. 42:45–66.
Westphal O, Jann K. 1965. Bacterial lipopolysaccharide. Extraction with phenol-water and further application of the procedure, In: Whistler RL (ed.), Methods in Carbohydrate Chemistry, Vol. 5, Academic Press, Inc., New York, pp. 83–91.
Whatley MH, Margot JB, Schell J, Lippincott BB, Lippincott JA. 1978. Plasmid and chromosomal determination of Agrobacterium adherence specificity. J. Gen. Microbiol. 107:393–396.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Martinus Nijhoff Publishers, Dordrecht
About this chapter
Cite this chapter
Expert, D., Schoonejans, E., Toussaint, A. (1987). Possible Involvement of Outer Membrane Components in Erwinia Chrysanthemi Pathogenicity. In: Civerolo, E.L., Collmer, A., Davis, R.E., Gillaspie, A.G. (eds) Plant Pathogenic Bacteria. Current Plant Science and Biotechnology in Agriculture, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3555-6_26
Download citation
DOI: https://doi.org/10.1007/978-94-009-3555-6_26
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8090-3
Online ISBN: 978-94-009-3555-6
eBook Packages: Springer Book Archive