Abstract
Enhancement of photosynthesis by supplemental photosystem 1-enriched (707nm) light has been investigated in intact spinach chloroplasts by the simultaneous measurement of the rate of oxygen evolution, yield of chlorophyll fluorescence and quenching of 9-aminoacridine fluorescence. Chloroplasts reducing CO2 showed a 75% increase in the rate of O2 evolution after the addition of 707nm light, whereas if nitrite was used as substrate, an enhancement of only 20% was observed. Reduction of glycerate-3-phosphate was associated with a 40% enhancement by 707nm light. There appears to be a correlation between the degree of enhancement and the requirement for ATP in addition to reducing power. Prolonged illumination in 707nm light resulted in an elevation of enhancement whereas illumination with 650nm light caused a loss of enhancement, demonstrating the operation of state transitions in intact isolated chloroplasts.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Arnon DI (1977) In: Encyclopedia of Plant Physiol Photosynthesis 1 (Trebst, A. and Avron, M. eds.) vol.V, pp 7–56, Springer Verlag, Berlin.
Bonaventura C and Myers J (1969) Biochim Biophys Acta 189: 366–383.
Canaani O, Barber J and Malkin S (1984) Proc Natl Acad Sc 81: 1614–1618.
Chow WS, Telfer A, Chapman DJ and Barber J (1981) Biochim Biophys Acta 638: 60–68.
Fernyhough P, Foyer CH and Horton P (1983) Biochim Biophys Acta 725: 155–161.
Fernyhough P, Foyer CH and Horton P (1984) FEBS Lett 176: 133–138.
Govindjee, Govindjee R and Hoch G (1964) Plant Physiol 39: 10–14.
Horton P and Black MT (1981) FEBS Lett 125: 193–196.
Horton P (1983a) FEBS Lett 152: 47–52.
Horton P (1983b) Proc Roy Soc Lond B 217: 405–415.
Horton P (1985a) In: Topics in Photosynthesis. Photosynthetic Mechanisms and the Environment (Barber, J. and Baker, N.R. eds.) pp 135–187 Elsevier Biomedical Press.
Horton P (1985b) In: Regulation of Sources and Sinks in Crop Plants (Jeffcoat, B.,Hawkins, A.F. and Stead, A.D. eds.) pp 19–33 Brit PI Growth Reg Group, Bristol.
Joliot P, Joliot A and Kok B (1968) Biochim Biophys Acta 153: 635–652.
McSwain BD and Arnon DI (1968) Proc Natl Acad Sei USA 61: 989–996.
McSwain BD and Arnon DI (1972) Biochem Biophys Res Commun 49: 68–75.
Myers J (1971) Ann Rev Plant Physiol 22: 289–312.
17.Sinclair J (1972) Plant Physiol 50: 778–783.
Sun ASK (1972) Biochim Biophys Acta 256: 409–427.
Takahama U, Shimizu-Takahama M and Heber U (1981) Biochim Biophys Acta 637: 530–539.
Telfer A, Allen JF, Barber J and Bennett J (1983) Biochim Biophys Acta 722: 176–181.
Walker DA (1971) Methods in Enzymology 23: 211–220.
Walker DA (1973) New Phytologist 72: 209–235.
Williams WP, Salaman Z, Muallem A, Barber J and Mills J (1976) Biochim Biophys Acta 430: 300–311.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Martinus Nijhoff Publishers, Dordrecht
About this chapter
Cite this chapter
Horton, P., Lee, P. (1987). Observation of Enhancement and State Transitions in Isolated Intact Chloroplasts. In: Govindjee, et al. Excitation Energy and Electron Transfer in Photosynthesis. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3527-3_18
Download citation
DOI: https://doi.org/10.1007/978-94-009-3527-3_18
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8076-7
Online ISBN: 978-94-009-3527-3
eBook Packages: Springer Book Archive