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dsRNA killer systems in yeast

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Yeast Biotechnology

Abstract

Killer yeasts, first identified by Bevan and Makower (1963), secrete proteinaceous toxins which kill other, sensitive yeasts. It is now more than a decade since the cytoplasmic determinants associated with the killing phenomenon were shown to be encapsidated dsRNA molecules. It was originally demonstrated that only two major species of dsRNA molecule were involved — the L and M dsRNAs. Since then a plethora of literature has emerged identifying or reporting the existence of a whole range of dsRNA species. Some of these have been named, and are, in decreasing size order, XL, LBC and LA (co- or closely migrating species), T, W and M. Despite this apparent increase in the complexity of the system, there is evidence for only one L genome, LA, and the M genome being involved in the killer phenomenon. No evidence links the ‘other’ species, XL, LBC, T and W with the killer system. However, since these species have not, unlike the 5.9-kb encapsidated dsRNA intermediates of the yeast Ty elements, been implicated in any other system, they will be considered here. The involvement of LA and M genomes is beyond doubt. LA dsRNAs encode their own major capsid polypeptide, and that of M dsRNA. M dsRNAs encode the killer toxin and immunity function.

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  1. Diane J. Mitchell
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    D. R. Berry

  2. Labatt Brewing Co., London, Ontario, Canada

    I. Russell  & G. G. Stewart  & 

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Mitchell, D.J., Bevan, E.A. (1987). dsRNA killer systems in yeast. In: Berry, D.R., Russell, I., Stewart, G.G. (eds) Yeast Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3119-0_5

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