Abstract
Isolation of protoplasts from plant cells involves a number of steps, the impact of which on the physiological response of the resulting protoplasts is not well understood. In particular, the usual treatment of cells with hydrolytic enzymes of fungal origin has received little attention, except for empirical selection of appropriate commercial preparations and, in some cases, their purification via gel filtration.
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References
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© 1988 Kluwer Academic Publishers
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Hahne, G., Lörz, H. (1988). Release of Phytotoxic Factors from Plant Cell Walls during Protoplast Isolation. In: Puite, K.J., Dons, J.J.M., Huizing, H.J., Kool, A.J., Koornneef, M., Krens, F.A. (eds) Progress in Plant Protoplast Research. Current Plant Science and Biotechnology in Agriculture, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2788-9_48
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DOI: https://doi.org/10.1007/978-94-009-2788-9_48
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7754-5
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