Abstract
In this paper we describe preliminary results from the development and characterization of a fiber optic biosensor that utilizes a fluorescently-labeled monoclonal antibody to pyrethroid pesticides. A synthetic pyrethroid-like antigen was coupled to bovine serum albumin and immobilized on the surface of a stripped portion of the distal end of an optical fiber. Each fiber was individually calibrated for its optical response using standard solutions of fluoresceinamine. Binding of the antibody was detected via the evanescent wave of the fiber by use of a portable fiber optic fluorimeter. Initial binding rate and equilibrium levels of bound antibody showed a linear relationship with antibody concentrations between 7 nM and 25 uM. To test the reversibility of the biosensor, bound antibody was removed with protein denaturing agents and the same fibers were re-tested with similar concentrations of antibody. When re-immersed in antibody solution, binding of the antibodies was again observed. The results provide encouraging evidence that such a biosensor may be useful for the determination of small organic molecules in situ
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Andrade, J.D., Vanwagenen, R.A., Gregonis, D.E., Newby, K., and Lin, J.N., IEEE Trans. Electr. Dev., ED-32, (7), (1985), 1175.
Brown, J., In Compound Evaluation and Analytical Capability National, Residue Program Plan, United States Department of Agriculture Food Safety and Inspection Service; Washington, (1987).
Hirschfeld, T.E., Can. Spectrosc., Nov., (1965), 126.
Janata, J. and Bezegh, A, Anal. Chem., 60 (12), (1988), 62R.
Kohler, G. and Milstein, Nature, 256, (1975), 4953.
Lynn, M., In Immobilized Enzymes, Antigens, Antibodies, and Peptides: Preparation and Chromatography, H.H. Weetall (Ed.), Marcel Dekker Inc., New York, (1975), pp 1–48.
Kronick, M. N. and Little, W.A., J. Immunol. Meth., 8, (1975), 235.
Liu, B.L., and Shultz, J.S., IEEE Trans. Biomed. Engin., BME-33, (2), (1986), 13
Milanovich, F., Garvis, D., Angel, S.M., Klainer, S.M. and Eccles, L., Anal. Inst., 15, (1986), 137.
Stanker, L.H., Bigbee, C, van Emon, J., Watkins, B., Jensen, R.H., Morris, C., and Vanderlaan, M., J. Agri. and Food Chem. In Press.
Sutherland, R.M., Dahne, C., Place, J., and Ringose, A.S., Clin. Chem., 30, (9), (1984), 1533.
The, T.E., and Feltknamp, T.E.W., Immunology, 18, (1970), 865.
Tromberg, B.J., Sepaniak, M.J., Alarie, J.P., Vo-Dinh, T., and Santella, R.M., Anal. Chem., 60, (1988), 1901.
Tromberg, B.J., Sepaniak, M.J., Vo-Dinh, T., and Griffin, G.D., Anal. Chem., 59, (1987), 1226.
Vanderlaan, M., Watkins, B., and Stanker, L. Environ. Sci. and Technol., 22, (1988), 247.
Vanwagenen, R.A. and Andrade, J.D., Fed. Proc., 41 (7074), (1982)
Weetall, H.H., In Immobilized Biochemicals and Affinity Chromatography, R.B. Dunlap (Ed.), Plenum Press, New York, (1974), pp 191–212.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Kluwer Academic Publishers
About this chapter
Cite this chapter
Northrup, M.A., Stanker, L.H., Vanderlaan, M., Watkins, B.E. (1989). Development and Characterization of a Fiber Optic Immuno-Biosensor. In: Theophanides, T. (eds) Spectroscopy of Inorganic Bioactivators. NATO ASI Series, vol 280. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2409-3_5
Download citation
DOI: https://doi.org/10.1007/978-94-009-2409-3_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7585-5
Online ISBN: 978-94-009-2409-3
eBook Packages: Springer Book Archive