Recovery of Cellulases From a Fermentation Broth

Abstract

Cellulases are an enzymatic system that can be used to maximize some technological procedures in Food and Feed industry. With Tricho-derma reesei cellulases obtained at controlled conditions the isolation of the enzymatic system was achieved using anion exchange on DEAE-Sephadex, cation exchange on SE-Sephadex and gelfiltration. Molecular weights were determined by SDS-Page and gelfiltration on Sephacryl S-200. Molecular weights of 4 endoglucanases, 1 cellobiohydrolase 1 β-glucosidase are presented. The recovery of these enzymes from fermentation broth at an apropriate concentration is made by ultrafiltration, on a hollow fiber device. The increase of process time produces a higher concentration factor though the specific enzymatic activity decreases deeply at a constant rate.

The optimization is achieved by the application of response surface methodology, planning the number of the experiments for a defined domain.

Future prospects on computer simulating conditions will improve the competitivity of the biological technologies.