Abstract
Procedures for the routine regeneration of large numbers of plants from isolated microspore cultures of maize have been recently developed (1,2). Haploid microspores are competent to undergo abnormal cell divisions culminating in the formation of embryo-like structures from which plants can be recovered. These procedures have now been adapted for the production of suspension and protoplast cultures capable of plant regeneration. The present paper describes the establishment of regenerable, haploid cell suspensions directly from cultured maize microspores. The recovery of plants from protoplasts isolated from these microspore-derived suspension cultures is also discussed.
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© 1990 Kluwer Academic Publishers
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Mitchell, J.C., Petolino, J.F. (1990). Haploid Suspension and Protoplast Culture from Isolated Microspores of Maize. In: Nijkamp, H.J.J., Van Der Plas, L.H.W., Van Aartrijk, J. (eds) Progress in Plant Cellular and Molecular Biology. Current Plant Science and Biotechnology in Agriculture, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2103-0_40
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DOI: https://doi.org/10.1007/978-94-009-2103-0_40
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7445-2
Online ISBN: 978-94-009-2103-0
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