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Antigenic Properties of the Ehrlichiae and other Rickettsiaceae

  • Chapter
Ehrlichiosis

Part of the book series: Current Topics in Veterinary Medicine and Animal Science ((CTVM,volume 54))

Abstract

The family Rickettsiaceae consists of a diverse group of small gram-negative bacteria, most of which show intimate association with diverse arthropods and exhibit obligate intracellular growth. The chemical and antigenic properties of members of the tribe Rickettsieae (Rickettsia, Rochalimaea, Coxiella) are understood relatively well in contrast to those of the tribe Ehrlichieae (Ehrlichia, Cowdria, Neorickettsia) or the poorly known tribe Wolbachieae (Wolbachia, Rickettsiella). With the advent of improved in-vitro cultivation systems for an increasing number of Rickettsiaceae, quality preparations of these diverse bacteria can now be obtained routinely by procedures such as Renografin or Percoll density gradient centrifugation. The availability of purified preparations has permitted the initiation of studies of the molecular and antigenic properties of the unique genus- and species-specific antigens. We present a brief review of the Rickettsiaceae with regard to current progress achieved in the chemical characterization of purified lipopolysaccharide and protein antigens, Western blotting comparisons of antigens with both polyclonal and monoclonal antibodies, and the molecular cloning and sequencing of DNA encoding different protein antigens. The phylogenetic implications for the Rickettsiaceae of the determination of the sequences of the conserved 60-kDa heat-shock proteins (Hsp 60) and 16S ribosomal RNAs are also considered.

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Authors
  1. G. A. Dasch Ph.D.
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  2. E. Weiss Ph.D.
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  3. J. C. Williams Ph.D.
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Editor information

Editors and Affiliations

  1. Office of the Director of Intramural Research Programs, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA

    Jim C. Williams Ph.D.

  2. Bacteriology Division, Department of Intracellular Pathogens, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA

    Jim C. Williams Ph.D.

  3. Department of Veterinary Pathobiology and Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, USA

    Ibulaimu Kakoma D.V.M., Ph.D.

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© 1990 Kluwer Academic Publishers

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Dasch, G.A., Weiss, E., Williams, J.C. (1990). Antigenic Properties of the Ehrlichiae and other Rickettsiaceae. In: Williams, J.C., Kakoma, I. (eds) Ehrlichiosis. Current Topics in Veterinary Medicine and Animal Science, vol 54. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1998-3_4

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  • DOI: https://doi.org/10.1007/978-94-009-1998-3_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7394-3

  • Online ISBN: 978-94-009-1998-3

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