Abstract
In the present study non-5-HT1A/non-5-HT1B binding sites in the rabbit caudate nucleus (CN) were examined to determine if they might be identical to the recently discovered 5-HT1D sites in the bovine CN. The characterizations were carried out measuring high-affinity [3H]5-HT binding under conditions where 5-HT1A and 5-HT1C sites were pharmacologically masked in both tissues. Comparison of the pharmacologic profiles of the bovine 5-HT1D and rabbit non-5-HT1A /non-5-HT1B sites revealed similarities, but showed distinct differences. For example, spiperone gave a Ki value of 2.8 μM in the rabbit CN, while concentrations of up to 100 μM did not produce even 50% inhibition of [3H]5-HT binding in the bovine CN. [3H]5-HT binding in the bovine CN was significantly more sensitive to inhibition by GTP than in the rabbit CN, and this effect was differentially sensitive to calcium in the two tissues. It was concluded that the non-5-HT1A /non-5-HT1B [3H]5-HT binding sites in rabbit CN are distinct from those in the bovine CN, and we propose that they be tentatively identified as 5-HT1R to distinguish them from the 5-HT1D site.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Fozard J. R. (1987) 5-HT: The enigma variations. Trends Pharmacol. Sci. 8, 501–506.
Peroutka S. J. (1988) 5-Hydroxytryptamine receptor subtypes., in Ann. Rev. Neurosci. Vol. 11, pp. 45–60. Annual Reviews, Inc.
Dumuis A., Bouhelal R., Sebben M., Cory R., and Bockaert, J. (1988) A nonclassical 5-hydroxytryptamine receptor positively coupled with adenylate cyclase in the central nervous system. Mol. Pharmacol. 34, 880–887.
Pierce P. A., and Peroutka S. J. (1989) Evidence for distinct 5- hydroxytryptamine2 binding site subtypes in cortical membrane preparations. J. Neurochem. 52, 656–658.
Pritchett D. B., Bach W. J., Wozny M., Taleb O., Dal Toso R., Shih J. C., and Seeburg P. H. (1988) Structure and functional expression of cloned rat serotonin 5HT-2 receptor. EMBO J. 7, 4135–4140.
Hartig P. R. (1989) Molecular biology of 5-HT receptors. Trends Pharmacol. Sci. 10, 64–69.
Richardson B. P., and Engel G. (1986) The pharmacology and function of 5-HT3 receptors. Trends Neurosci. 9, 424–428.
Schnellmann R. G., Waters S. J., and Nelson D. L. (1984) [3H]5- Hydroxytryptamine binding sites: species and tissue variation. J. Neurochem. 42, 65–70.
Heuring R. E., and Peroutka S. J. (1987) Characterization of a novel 3H-5-hydroxytryptamine binding site subtype in bovine brain membranes. J. Neurosci. 7, 894–903.
Pedigo N. W., Yamamura H. I., and Nelson D. L. (1981) Discrimination of multiple [3H] 5-hydroxytryptamine binding sites by the neuroleptic spiperone in rat brain. J. Neurochem. 36, 220–226.
Nelson D. L., Herbet A., Bourgoin S., Glowinski J., and Hamon M. (1978) Characteristics of central 5-HT receptors and their adaptive changes following intracerebral 5,7-dihydroxytryptamine administration in the rat. Mo. Pharmacol. 14, 983–995.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Kluwer Academic Publishers
About this chapter
Cite this chapter
Nelson, D.L., Xiong, WC. (1990). Classification of Serotonergic Receptors: An Example of a Novel 5-HT1-Like Binding Site in the Rabbit Caudate Nucleus. In: Paoletti, R., Vanhoutte, P.M., Brunello, N., Maggi, F.M. (eds) Serotonin. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1912-9_41
Download citation
DOI: https://doi.org/10.1007/978-94-009-1912-9_41
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7353-0
Online ISBN: 978-94-009-1912-9
eBook Packages: Springer Book Archive